Literature DB >> 18998045

Efficient production of type 2 porcine circovirus-like particles by a recombinant baculovirus.

Lan-Jun Liu1, Takako Suzuki, Hiroshi Tsunemitsu, Michiyo Kataoka, Noriyo Ngata, Naokazu Takeda, Takaji Wakita, Tatsuo Miyamura, Tian-Cheng Li.   

Abstract

The capsid protein of PCV2 was expressed by using a recombinant baculovirus with insect Tn5 cells. A large amount of 28-kDa protein was released into the culture medium and self-assembled into PCV2-like particles (PCV2-LPs) with a buoyant density of 1.365 g/cm(3) and a diameter of 20 nm. PCV2-LPs were efficiently expressed, yielding 1 mg of purified particles per 10(7) Tn5 cells. The PCV2-LPs have antigenicity similar to that of authentic PCV2 particles, allowing us to develop a method for sensitively detecting PCV2-specific IgG antibodies. In addition, the PCV2-LPs appeared to be the most promising PCV2 vaccine candidate, by virtue of their potent immunogenicity.

Entities:  

Mesh：

Substances：

Year:  2008        PMID： 18998045     DOI： 10.1007/s00705-008-0248-x

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  13 in total

1.  The Carboxyl Terminus of the Porcine Circovirus Type 2 Capsid Protein Is Critical to Virus-Like Particle Assembly, Cell Entry, and Propagation.

Authors:  Yang Zhan; Wanting Yu; Xiong Cai; Xinnuo Lei; Hongyu Lei; Aibing Wang; Yujie Sun; Naidong Wang; Zhibang Deng; Yi Yang
Journal:  J Virol       Date:  2020-04-16       Impact factor: 5.103

2.  In silico analysis of surface structure variation of PCV2 capsid resulting from loop mutations of its capsid protein (Cap).

Authors:  Naidong Wang; Yang Zhan; Aibing Wang; Lijie Zhang; Reza Khayat; Yi Yang
Journal:  J Gen Virol       Date:  2016-10-18       Impact factor: 3.891

3.  Recognition of the different structural forms of the capsid protein determines the outcome following infection with porcine circovirus type 2.

Authors:  Benjamin R Trible; Andrew W Suddith; Maureen A Kerrigan; Ada G Cino-Ozuna; Richard A Hesse; Raymond R R Rowland
Journal:  J Virol       Date:  2012-10-03       Impact factor: 5.103

4.  2.9 Å Resolution Cryo-EM 3D Reconstruction of Close-Packed Virus Particles.

Authors:  Zheng Liu; Fei Guo; Feng Wang; Tian-Cheng Li; Wen Jiang
Journal:  Structure       Date:  2016-01-14       Impact factor: 5.006

Review 5.  Recent vaccine technology in industrial animals.

Authors:  Hyunil Kim; Yoo-Kyoung Lee; Sang Chul Kang; Beom Ku Han; Ki Myung Choi
Journal:  Clin Exp Vaccine Res       Date:  2016-01-27

6.  Immunogenicity of plant-produced porcine circovirus-like particles in mice.

Authors:  Cornelius J Gunter; Guy L Regnard; Edward P Rybicki; Inga I Hitzeroth
Journal:  Plant Biotechnol J       Date:  2019-03-10       Impact factor: 9.803

7.  Heterologous expression of full-length capsid protein of porcine circovirus 2 in Escherichia coli and its potential use for detection of antibodies.

Authors:  Zuzana Marcekova; Ivan Psikal; Eva Kosinova; Oldrich Benada; Peter Sebo; Ladislav Bumba
Journal:  J Virol Methods       Date:  2009-08-05       Impact factor: 2.014

8.  Virus-like particles of chimeric recombinant porcine circovirus type 2 as antigen vehicle carrying foreign epitopes.

Authors:  Huawei Zhang; Ping Qian; Lifeng Liu; Suhong Qian; Huanchun Chen; Xiangmin Li
Journal:  Viruses       Date:  2014-12-05       Impact factor: 5.048

9.  Recombinant expression of beak and feather disease virus capsid protein and assembly of virus-like particles in Nicotiana benthamiana.

Authors:  Guy L Regnard; Edward P Rybicki; Inga I Hitzeroth
Journal:  Virol J       Date:  2017-09-11       Impact factor: 4.099

10.  The level of decoy epitope in PCV2 vaccine affects the neutralizing activity of sera in the immunized animals.

Authors:  Junyeong Jin; Changhoon Park; Sun-Hee Cho; Junho Chung
Journal:  Biochem Biophys Res Commun       Date:  2018-01-31       Impact factor: 3.575
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.