Literature DB >> 18997028

Impact of inactivated extracellular proteases on the modified flagellin type III secretion pathway of Bacillus halodurans.

Eldie Berger1, Erika du Plessis, Isak Gerber, Michael Crampton, Nolwandle Nxumalo, Maureen Louw.   

Abstract

The flagellin type III secretion pathway of Bacillus halodurans BhFC01 (Deltahag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as flagellin fusion monomers. The stability of the secreted monomers was significantly enhanced through gene-targeted inactivation of extracellular proteases.

Entities:  

Mesh：

Substances：

Year:  2008        PMID： 18997028      PMCID： PMC2612215          DOI： 10.1128/AEM.02430-08

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  10 in total

1.  Translation/secretion coupling by type III secretion systems.

Authors:  J E Karlinsey; J Lonner; K L Brown; K T Hughes
Journal:  Cell       Date:  2000-08-18       Impact factor: 41.582

2.  Cloning of the flagellin gene from Bacillus subtilis and complementation studies of an in vitro-derived deletion mutation.

Authors:  E R LaVallie; M L Stahl
Journal:  J Bacteriol       Date:  1989-06       Impact factor: 3.490

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates.

Authors:  C Heussen; E B Dowdle
Journal:  Anal Biochem       Date:  1980-02       Impact factor: 3.365

5.  Extracellular secretion of polypeptides using a modified Escherichia coli flagellar secretion apparatus.

Authors:  Katariina Majander; Lena Anton; Jenni Antikainen; Hannu Lång; Mirko Brummer; Timo K Korhonen; Benita Westerlund-Wikström
Journal:  Nat Biotechnol       Date:  2005-04-03       Impact factor: 54.908

6.  The development of a flagellin surface display expression system in a moderate thermophile, Bacillus halodurans Alk36.

Authors:  Michael Crampton; Eldie Berger; Sharon Reid; Maureen Louw
Journal:  Appl Microbiol Biotechnol       Date:  2007-02-21       Impact factor: 4.813

7.  Improvement of Bacillus sphaericus toxicity against dipteran larvae by integration, via homologous recombination, of the Cry11A toxin gene from Bacillus thuringiensis subsp. israelensis.

Authors:  S Poncet; C Bernard; E Dervyn; J Cayley; A Klier; G Rapoport
Journal:  Appl Environ Microbiol       Date:  1997-11       Impact factor: 4.792

8.  Functional production and characterization of a fibrin-specific single-chain antibody fragment from Bacillus subtilis: effects of molecular chaperones and a wall-bound protease on antibody fragment production.

Authors:  Sau-Ching Wu; Jonathan C Yeung; Yanjun Duan; Ruiqiong Ye; Steven J Szarka; Hamid R Habibi; Sui-Lam Wong
Journal:  Appl Environ Microbiol       Date:  2002-07       Impact factor: 4.792

9.  High-efficiency gene inactivation and replacement system for gram-positive bacteria.

Authors:  I Biswas; A Gruss; S D Ehrlich; E Maguin
Journal:  J Bacteriol       Date:  1993-06       Impact factor: 3.490

10.  Peptide immunogens based on the envelope region of HIV-1 are recognized by HIV/AIDS patient polyclonal antibodies and induce strong humoral immune responses in mice and rabbits.

Authors:  R Hewer; D Meyer
Journal:  Mol Immunol       Date:  2003-10       Impact factor: 4.407
  10 in total
  2 in total

1.  Extracellular secretion of a recombinant therapeutic peptide by Bacillus halodurans utilizing a modified flagellin type III secretion system.

Authors:  Eldie Berger; Michael C Crampton; Nolwandle P Nxumalo; Maureen E Louw
Journal:  Microb Cell Fact       Date:  2011-08-04       Impact factor: 5.328

2.  Inactivation of aprE Gene in Bacillus subtilis 168 by Homologus Recombination.

Authors:  Mohammed Rabbani; Safoura Soleymani; Hamid Mir Mohammad Sadeghi; Narjes Soleimani; Fatemeh Moazen
Journal:  Avicenna J Med Biotechnol       Date:  2014-07
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.