| Literature DB >> 18996642 |
Yasuyuki Gen1, Kohichiroh Yasui, Keika Zen, Tomoaki Nakajima, Kazuhiro Tsuji, Mio Endo, Hironori Mitsuyoshi, Masahito Minami, Yoshito Itoh, Shinji Tanaka, Masafumi Taniwaki, Shigeki Arii, Takeshi Okanoue, Toshikazu Yoshikawa.
Abstract
RhoA, a member of the Rho family of small GTPases, directs the organization of the actin cytoskeleton and is involved in regulating cell shape and movement. Its activity is negatively regulated by p190-B RhoGAP (GTPase-activating protein). We investigated DNA copy number aberrations in human hepatocellular carcinoma and esophageal squamous cell carcinoma cell lines using a high-density oligonucleotide microarray and found a novel amplification at chromosomal region 14q12. We identified ARHGAP5 (the gene encoding p190-B RhoGAP) as a probable target for the amplification at 14q12, and our results showed that p190-B RhoGAP promotes cells spreading and migration by negatively regulating RhoA activity in Huh-7 hepatocellular carcinoma cells.Entities:
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Year: 2008 PMID： 18996642 DOI： 10.1016/j.canlet.2008.09.036
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679