| Literature DB >> 18996215 |
Simona Passeri1, Francesca Nocchi, Roberta Lamanna, Simone Lapi, Vincenzo Miragliotta, Elisabetta Giannessi, Francesca Abramo, Maria Rita Stornelli, Micheletino Matarazzo, Daniele Plenteda, Patrizia Urciuoli, Fabrizio Scatena, Alessandra Coli.
Abstract
Stem cells from extra-embryonic sources can be obtained by non-invasive procedures. We have standardized a method for the expansion of equine umbilical cord-derived matrix cells (EUCMCs) for potential therapy. EUCMCs were isolated from the umbilical cord of five mares immediately after delivery. For expansion, cells were grown in alpha-MEM and MSCBM. Moreover, to measure the effect of growth factor supplementation, epidermal growth factor (EGF) was added to alpha-MEM. alpha-MEM and MSCBM media performed similarly in terms of population doubling and CFU number value. EGF supplementation of alpha-MEM determined a significant increase of the population doubling value. EGF supplementation did not affect the adipogenic and chondrogenic differentiation while bone nodule sizes an increased with the osteogenic protocol. Both alpha-MEM and MSCBM can be used to cultivate EUCMCs. alpha-MEM supplemented with EGF might represent an advantage for EUCMCs expansion. The results could be useful in choosing the culture medium since alpha-MEM is more cost-effective than MSCBM.Entities:
Mesh：
Substances：
Year: 2008 PMID： 18996215 DOI： 10.1016/j.cellbi.2008.10.012
Source DB: PubMed Journal: Cell Biol Int ISSN： 1065-6995 Impact factor: 3.612