Raphael Gorodetsky1. 1. Laboratory of Radiobiology and Biotechnology, Sharett Institute of Oncology, Hadassah-Hebrew University Medical Center, POB 12,000, Jerusalem, 91120, Israel. rafi@hadassah.org.il
Abstract
BACKGROUND: Due to its good cell attachment capabilities and promotion of cell migration, fibrin serves as an interim cell-binding matrix in wounded tissues. Due to their fast degradation, unprocessed fibrin matrices have limited use in tissue engineering. OBJECTIVE: To describe stable fibrin-based matrices for isolation, growth and delivery of stem cells for implantation to enhance tissue regeneration. METHODS: Fibrin microbeads (FMB) were produced by moderate-heat condensation of fibrin particles in oil without compromising the cell binding capability of the fibrin. RESULTS: Mesenchymal stem cells (MSC) were separated from different sources at much higher yields with FMB. They were further expanded on them in suspension without trypsinization and passages. Cells on FMB could be induced to differentiate into different phenotypes, such as bone and cartilage. This enabled implantation of the cells on FMB for cell-based tissue regeneration. CONCLUSIONS: FMB technology provides a simple and effective method for cell separation, expansion in suspension and delivery for tissue regeneration.
BACKGROUND: Due to its good cell attachment capabilities and promotion of cell migration, fibrin serves as an interim cell-binding matrix in wounded tissues. Due to their fast degradation, unprocessed fibrin matrices have limited use in tissue engineering. OBJECTIVE: To describe stable fibrin-based matrices for isolation, growth and delivery of stem cells for implantation to enhance tissue regeneration. METHODS: Fibrin microbeads (FMB) were produced by moderate-heat condensation of fibrin particles in oil without compromising the cell binding capability of the fibrin. RESULTS: Mesenchymal stem cells (MSC) were separated from different sources at much higher yields with FMB. They were further expanded on them in suspension without trypsinization and passages. Cells on FMB could be induced to differentiate into different phenotypes, such as bone and cartilage. This enabled implantation of the cells on FMB for cell-based tissue regeneration. CONCLUSIONS: FMB technology provides a simple and effective method for cell separation, expansion in suspension and delivery for tissue regeneration.
Authors: Helena Escuin-Ordinas; Shuoran Li; Michael W Xie; Lu Sun; Willy Hugo; Rong Rong Huang; Jing Jiao; Felipe Meira de-Faria; Susan Realegeno; Paige Krystofinski; Ariel Azhdam; Sara Marie D Komenan; Mohammad Atefi; Begoña Comin-Anduix; Matteo Pellegrini; Alistair J Cochran; Robert L Modlin; Harvey R Herschman; Roger S Lo; William H McBride; Tatiana Segura; Antoni Ribas Journal: Nat Commun Date: 2016-08-01 Impact factor: 14.919
Authors: Sergei A Kuznetsov; Astar Hailu-Lazmi; Natasha Cherman; Luis F de Castro; Pamela G Robey; Raphael Gorodetsky Journal: Stem Cells Transl Med Date: 2019-02-14 Impact factor: 6.940
Authors: Michael W Xie; Raphael Gorodetsky; Ewa D Micewicz; Ewa D Micevicz; Natalia C Mackenzie; Elena Gaberman; Lilia Levdansky; William H McBride Journal: J Invest Dermatol Date: 2012-09-06 Impact factor: 8.551