Literature DB >> 18989440

Micro-dissection of rat brain for RNA or protein extraction from specific brain region.

Kin Chiu1, Wui Man Lau, Ho Tak Lau, Kwok-Fai So, Raymond Chuen-Chung Chang.   

Abstract

Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates micro-dissection of four major brain regions include olfactory bulb, frontal cortex, striatum and hippocampus in fresh rat brain tissue. Useful tips for quick removal of respective regions to avoid RNA and protein degradation of the tissue are given.

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Year:  2007        PMID： 18989440      PMCID： PMC2565859          DOI： 10.3791/269

Source DB:  PubMed          Journal:  J Vis Exp        ISSN： 1940-087X            Impact factor:   1.355


  49 in total

1.  Ozone modulates the effects of imipramine on immobility in the forced swim test, and nonspecific parameters of hippocampal oxidative stress in the rat.

Authors:  Mmalebuso L Mokoena; Brian H Harvey; Douglas W Oliver; Christiaan B Brink
Journal:  Metab Brain Dis       Date:  2010-05-09       Impact factor: 3.584

2.  In-depth neuropharmacokinetic analysis of antipsychotics based on a novel approach to estimate unbound target-site concentration in CNS regions: link to spatial receptor occupancy.

Authors:  I Loryan; E Melander; M Svensson; M Payan; F König; B Jansson; M Hammarlund-Udenaes
Journal:  Mol Psychiatry       Date:  2016-01-26       Impact factor: 15.992

3.  Differential Effect of Caffeine Consumption on Diverse Brain Areas of Pregnant Rats.

Authors:  Inmaculada Ballesteros-Yáñez; Carlos Alberto Castillo; Mariano Amo-Salas; José Luis Albasanz; Mairena Martín
Journal:  J Caffeine Res       Date:  2012-06

4.  Analysis of neurotransmitter levels in addiction-related brain regions during synthetic cathinone self-administration in male Sprague-Dawley rats.

Authors:  Julie A Marusich; Elaine A Gay; Bruce E Blough
Journal:  Psychopharmacology (Berl)       Date:  2018-09-06       Impact factor: 4.530

5.  Large-Scale, Ion-Current-Based Proteomic Investigation of the Rat Striatal Proteome in a Model of Short- and Long-Term Cocaine Withdrawal.

Authors:  Shichen Shen; Xiaosheng Jiang; Jun Li; Robert M Straubinger; Mauricio Suarez; Chengjian Tu; Xiaotao Duan; Alexis C Thompson; Jun Qu
Journal:  J Proteome Res       Date:  2016-04-11       Impact factor: 4.466

6.  Excess folate during adolescence suppresses thyroid function with permanent deficits in motivation and spatial memory.

Authors:  L J Sittig; L B K Herzing; H Xie; K K Batra; P K Shukla; E E Redei
Journal:  Genes Brain Behav       Date:  2011-12-13       Impact factor: 3.449

7.  Electroconvulsive Seizures in Rats and Fractionation of Their Hippocampi to Examine Seizure-induced Changes in Postsynaptic Density Proteins.

Authors:  Sung-Soo Jang; Han Gil Jeong; Hee Jung Chung
Journal:  J Vis Exp       Date:  2017-08-15       Impact factor: 1.355

8.  Genetic Deficiency of Complement Component 3 Does Not Alter Disease Progression in a Mouse Model of Huntington's Disease.

Authors:  Paul B Larkin; Paul J Muchowski
Journal:  J Huntingtons Dis       Date:  2012

9.  Maternal separation with early weaning: a rodent model providing novel insights into neglect associated developmental deficits.

Authors:  Becky C Carlyle; Alvaro Duque; Robert R Kitchen; Kelly A Bordner; Daniel Coman; Eliza Doolittle; Xenophonios Papademetris; Fahmeed Hyder; Jane R Taylor; Arthur A Simen
Journal:  Dev Psychopathol       Date:  2012-11

10.  Dexamethasone Alters the Appetite Regulation via Induction of Hypothalamic Insulin Resistance in Rat Brain.

Authors:  Ragitha Chruvattil; Shreya Banerjee; Sarmi Nath; Jatin Machhi; Gitika Kharkwal; Mange Ram Yadav; Sarita Gupta
Journal:  Mol Neurobiol       Date:  2016-11-07       Impact factor: 5.590
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