| Literature DB >> 18987806 |
Fabio Mohn1, Michael Weber, Dirk Schübeler, Tim-Christoph Roloff.
Abstract
Methylated DNA immunoprecipitation (MeDIP) is a versatile immunocapturing approach for unbiased detection of methylated DNA. In brief, genomic DNA is randomly sheared by sonication and immunoprecipitated with a monoclonal antibody that specifically recognizes 5-methylcytidine. The resulting enrichment of methylated DNA in the immunoprecipitated fraction can be determined by PCR to assess the methylation state of individual regions. Alternatively, MeDIP can be combined with large-scale analysis using microarrays as a genome-wide experimental readout. This protocol has been applied to generate comprehensive DNA methylation profiles on a genome-wide scale in mammals and plants, and further to identify abnormally methylated genes in cancer cells.Entities:
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Year: 2009 PMID： 18987806 DOI： 10.1007/978-1-59745-522-0_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745