Literature DB >> 18987800

Is 2,3-butanedione monoxime an effective inhibitor of myosin-based activities in plant cells?

D W McCurdy1.   

Abstract

The effectiveness of 2,3-butanedione monoxime (BDM) as an inhibitor of plant myosins has been investigated. Three myosin-dependent motility phenomena in plants, namely cytoplasmic streaming in Chara corallina, light-dependent chloroplast repositioning in Elodea sp., and brefeldin A(BFA)-induced Golgi membrane dynamics in wheat (Triticum aestivum L. cv. Kite) root-tip cells were investigated. All three processes were inhibited by the sulfhydryl-modifying agent N-ethylmalemide (NEM), indicating the probable involvement of myosin as the motor protein in each case. However, none of these myosin-dependent processes were inhibited by BDM at concentrations as high as 20 mM in some instances. These results therefore question the general usefulness of BDM as an inhibitor of myosin-based activities in plant cells.

Entities:  

Year:  1999        PMID： 18987800     DOI： 10.1007/BF01415707

Source DB:  PubMed          Journal:  Protoplasma        ISSN： 0033-183X            Impact factor:   3.356


  11 in total

1.  Actomyosin-based motility of endoplasmic reticulum and chloroplasts in Vallisneria mesophyll cells.

Authors:  S Liebe; D Menzel
Journal:  Biol Cell       Date:  1995       Impact factor: 4.458

2.  Mechanisms of the contractile effects of 2,3-butanedione-monoxime in the mammalian heart.

Authors:  N Zimmermann; P Boknik; E Gams; S Gsell; L R Jones; R Maas; J Neumann; H Scholz
Journal:  Naunyn Schmiedebergs Arch Pharmacol       Date:  1996-10       Impact factor: 3.000

3.  Localization of a myosin-like protein to plasmodesmata.

Authors:  J E Radford; R G White
Journal:  Plant J       Date:  1998-06       Impact factor: 6.417

4.  Stimulation of radial expansion in arabidopsis roots by inhibitors of actomyosin and vesicle secretion but not by various inhibitors of metabolism.

Authors:  T I Baskin; N J Bivens
Journal:  Planta       Date:  1995       Impact factor: 4.116

5.  The myosin ATPase inhibitor 2,3-butanedione-2-monoxime (BDM) inhibits tip growth and cytokinesis in the fission yeast, Schizosaccharomyces pombe.

Authors:  K M May; S P Wheatley; V Amin; J S Hyams
Journal:  Cell Motil Cytoskeleton       Date:  1998

6.  Molecular analysis of the myosin gene family in Arabidopsis thaliana.

Authors:  M Kinkema; H Wang; J Schiefelbein
Journal:  Plant Mol Biol       Date:  1994-11       Impact factor: 4.076

7.  Novel suppression of an L-type calcium channel in neurones of murine dorsal root ganglia by 2,3-butanedione monoxime.

Authors:  G J Huang; J J McArdle
Journal:  J Physiol       Date:  1992-02       Impact factor: 5.182

8.  Butanedione monoxime suppresses contraction and ATPase activity of rabbit skeletal muscle.

Authors:  H Higuchi; S Takemori
Journal:  J Biochem       Date:  1989-04       Impact factor: 3.387

9.  The Saccharomyces cerevisiae MYO2 gene encodes an essential myosin for vectorial transport of vesicles.

Authors:  G C Johnston; J A Prendergast; R A Singer
Journal:  J Cell Biol       Date:  1991-05       Impact factor: 10.539

10.  Myosin is involved in postmitotic cell spreading.

Authors:  L P Cramer; T J Mitchison
Journal:  J Cell Biol       Date:  1995-10       Impact factor: 10.539
View more
  12 in total

1.  Cell wall pectins and xyloglucans are internalized into dividing root cells and accumulate within cell plates during cytokinesis.

Authors:  F Baluska; F Liners; A Hlavacka; M Schlicht; P Van Cutsem; D W McCurdy; D Menzel
Journal:  Protoplasma       Date:  2005-10-05       Impact factor: 3.356

2.  Myosins XI Are Involved in Exocytosis of Cellulose Synthase Complexes.

Authors:  Weiwei Zhang; Chao Cai; Christopher J Staiger
Journal:  Plant Physiol       Date:  2019-01-31       Impact factor: 8.340

3.  Arabidopsis myosin XI: a motor rules the tracks.

Authors:  Chao Cai; Jessica L Henty-Ridilla; Daniel B Szymanski; Christopher J Staiger
Journal:  Plant Physiol       Date:  2014-09-18       Impact factor: 8.340

4.  Inhibitors of myosin, but not actin, alter transport through Tradescantia plasmodesmata.

Authors:  Janine E Radford; Rosemary G White
Journal:  Protoplasma       Date:  2010-11-27       Impact factor: 3.356

5.  Myosin inhibitors block accumulation movement of chloroplasts in Arabidopsis thaliana leaf cells.

Authors:  H Paves; E Truve
Journal:  Protoplasma       Date:  2007-04-24       Impact factor: 3.356

6.  Structure and dynamics of an Arp2/3 complex-independent component of the lamellipodial actin network.

Authors:  John H Henson; David Cheung; Christopher A Fried; Charles B Shuster; Mary K McClellan; Meagen K Voss; John T Sheridan; Rudolf Oldenbourg
Journal:  Cell Motil Cytoskeleton       Date:  2009-09

7.  Rapid severing and motility of chloroplast-actin filaments are required for the chloroplast avoidance response in Arabidopsis.

Authors:  Sam-Geun Kong; Yoshiyuki Arai; Noriyuki Suetsugu; Toshio Yanagida; Masamitsu Wada
Journal:  Plant Cell       Date:  2013-02-12       Impact factor: 11.277

8.  Actin-dependent deposition of putative endosomes and endoplasmic reticulum during early stages of wound healing in characean internodal cells.

Authors:  A Klima; I Foissner
Journal:  Plant Biol (Stuttg)       Date:  2010-12-14       Impact factor: 3.081

9.  2,3-Butanedione monoxime (BDM) as a myosin inhibitor.

Authors:  E Michael Ostap
Journal:  J Muscle Res Cell Motil       Date:  2002       Impact factor: 3.352

10.  Actin filament dynamics are dominated by rapid growth and severing activity in the Arabidopsis cortical array.

Authors:  Christopher J Staiger; Michael B Sheahan; Parul Khurana; Xia Wang; David W McCurdy; Laurent Blanchoin
Journal:  J Cell Biol       Date:  2009-01-26       Impact factor: 10.539
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.