OBJECTIVE: Describe the presence of plasmacytoid dendritic cells and type 1 interferon and the activation of interferon signaling pathway in lymphatic malformations. DESIGN: Retrospective case series. MATERIALS: Nineteen lymphatic malformations with matching clinical data were compared to control tissue (normal skin and lymph node). METHODS: Paraffin-embedded tissue was evaluated for plasmacytoid dendritic cells, type 1 interferon, and type 1 interferon signaling with immunohistochemistry and RNA in situ hybridization. Staining results were compared with controls and correlated with clinical data with descriptive statistics and Mann-Whitney tests. RESULTS: Lymphatic malformations had increased staining for plasmacytoid dendritic cells, interferons alpha and beta, and phosphorylated signal transducer and activator of transcription 1 compared with controls (P < 0.05). Significantly increased interferon beta staining was present in recurrent lymphatic malformation tissue compared with nonrecurrent lymphatic malformations. CONCLUSION: Lymphatic malformations have unusual ongoing local immune activation, possibly affecting recurrence.
OBJECTIVE: Describe the presence of plasmacytoid dendritic cells and type 1 interferon and the activation of interferon signaling pathway in lymphatic malformations. DESIGN: Retrospective case series. MATERIALS: Nineteen lymphatic malformations with matching clinical data were compared to control tissue (normal skin and lymph node). METHODS:Paraffin-embedded tissue was evaluated for plasmacytoid dendritic cells, type 1 interferon, and type 1 interferon signaling with immunohistochemistry and RNA in situ hybridization. Staining results were compared with controls and correlated with clinical data with descriptive statistics and Mann-Whitney tests. RESULTS:Lymphatic malformations had increased staining for plasmacytoid dendritic cells, interferons alpha and beta, and phosphorylated signal transducer and activator of transcription 1 compared with controls (P < 0.05). Significantly increased interferon beta staining was present in recurrent lymphatic malformation tissue compared with nonrecurrent lymphatic malformations. CONCLUSION:Lymphatic malformations have unusual ongoing local immune activation, possibly affecting recurrence.
Authors: Andrew L Kirsh; Sharon L Cushing; Eunice Y Chen; Stephen M Schwartz; Jonathan A Perkins Journal: Lymphat Res Biol Date: 2011 Impact factor: 2.589
Authors: Christina Danial; Andrea L Tichy; Umar Tariq; Glenda L Swetman; Phuong Khuu; Thomas H Leung; Latanya Benjamin; Joyce Teng; Shreyas S Vasanawala; Alfred T Lane Journal: J Am Acad Dermatol Date: 2014-03-20 Impact factor: 11.527