Literature DB >> 1898328

Synthesis of a gene for rat liver fatty-acid-binding protein and its expression in Escherichia coli.

A F Worrall1, C Evans, D C Wilton.   

Abstract

A gene coding for rat liver fatty-acid-binding protein (FABP) has been constructed by the single-step ligation of ten synthetic oligonucleotides. The gene has been cloned into the bacterial expression vector pKK223-3. Induction of protein synthesis from this gene results in over expression of an FABP that is indistinguishable in its structure and binding properties with that isolated from rat liver.

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Year:  1991        PMID： 1898328      PMCID： PMC1151350          DOI： 10.1042/bj2780365

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  29 in total

1.  The chemical modification of cysteine-69 of rat liver fatty acid-binding protein (FABP): a fluorescence approach to FABP structure and function.

Authors:  C Evans; D C Wilton
Journal:  Mol Cell Biochem       Date:  1990 Oct 15-Nov 8       Impact factor: 3.396

2.  A comprehensive list of chemically synthesized genes.

Authors:  G Gröger; F Ramalho-Ortigao; H Steil; H Seliger
Journal:  Nucleic Acids Res       Date:  1988-08-25       Impact factor: 16.971

3.  The T7 phage gene 10 leader RNA, a ribosome-binding site that dramatically enhances the expression of foreign genes in Escherichia coli.

Authors:  P O Olins; C S Devine; S H Rangwala; K S Kavka
Journal:  Gene       Date:  1988-12-15       Impact factor: 3.688

4.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

5.  Improved oligonucleotide site-directed mutagenesis using M13 vectors.

Authors:  P Carter; H Bedouelle; G Winter
Journal:  Nucleic Acids Res       Date:  1985-06-25       Impact factor: 16.971

6.  The chemical synthesis of a gene coding for bovine pancreatic DNase I and its cloning and expression in Escherichia coli.

Authors:  A F Worrall; B A Connolly
Journal:  J Biol Chem       Date:  1990-12-15       Impact factor: 5.157

7.  Studies on fatty-acid-binding proteins. The purification of rat liver fatty-acid-binding protein and the role of cysteine-69 in fatty acid binding.

Authors:  D C Wilton
Journal:  Biochem J       Date:  1989-07-01       Impact factor: 3.857

8.  Refined apoprotein structure of rat intestinal fatty acid binding protein produced in Escherichia coli.

Authors:  J C Sacchettini; J I Gordon; L J Banaszak
Journal:  Proc Natl Acad Sci U S A       Date:  1989-10       Impact factor: 11.205

9.  The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.

Authors:  J Shine; L Dalgarno
Journal:  Proc Natl Acad Sci U S A       Date:  1974-04       Impact factor: 11.205

10.  The three-dimensional structure of P2 myelin protein.

Authors:  T A Jones; T Bergfors; J Sedzik; T Unge
Journal:  EMBO J       Date:  1988-06       Impact factor: 11.598
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  12 in total

1.  Comparison of the catalytic properties of phospholipase A2 from pancreas and venom using a continuous fluorescence displacement assay.

Authors:  A Kinkaid; D C Wilton
Journal:  Biochem J       Date:  1991-09-15       Impact factor: 3.857

Review 2.  The effect of charge reversal mutations in the alpha-helical region of liver fatty acid binding protein on the binding of fatty-acyl CoAs, lysophospholipids and bile acids.

Authors:  Robert M Hagan; Joanna K Davies; David C Wilton
Journal:  Mol Cell Biochem       Date:  2002-10       Impact factor: 3.396

3.  The properties of a cloned human high-molecular-mass cytosolic phospholipase A2 investigated using a continuous fluorescence displacement assay: evidence for enzyme clustering on phospholipid vesicles.

Authors:  A Creaney; D J Masters; M B Needham; R D Gordon; R Mott; D C Wilton
Journal:  Biochem J       Date:  1995-03-15       Impact factor: 3.857

4.  The binding of cholesterol and bile salts to recombinant rat liver fatty acid-binding protein.

Authors:  A E Thumser; D C Wilton
Journal:  Biochem J       Date:  1996-12-15       Impact factor: 3.857

5.  Rat liver mitochondrial phospholipase A2 is an endotoxin-stimulated membrane-associated enzyme of Kupffer cells which is released during liver perfusion.

Authors:  G M Hatch; D E Vance; D C Wilton
Journal:  Biochem J       Date:  1993-07-01       Impact factor: 3.857

6.  The binding of natural and fluorescent lysophospholipids to wild-type and mutant rat liver fatty acid-binding protein and albumin.

Authors:  A E Thumser; D C Wilton
Journal:  Biochem J       Date:  1995-04-01       Impact factor: 3.857

7.  The binding of lysophospholipids to rat liver fatty acid-binding protein and albumin.

Authors:  A E Thumser; J E Voysey; D C Wilton
Journal:  Biochem J       Date:  1994-08-01       Impact factor: 3.857

8.  Effect on ligand binding of arginine mutations in recombinant rat liver fatty acid-binding protein.

Authors:  A E Thumser; C Evans; A F Worrall; D C Wilton
Journal:  Biochem J       Date:  1994-01-01       Impact factor: 3.857

9.  Characterization of binding and structural properties of rat liver fatty-acid-binding protein using tryptophan mutants.

Authors:  A E Thumser; D C Wilton
Journal:  Biochem J       Date:  1994-06-15       Impact factor: 3.857

10.  Enhanced hydrolysis of phosphatidylcholine by human group II non-pancreatic secreted phospholipase A2 as a result of interfacial activation by specific anions. Potential role of cholesterol sulphate.

Authors:  A R Kinkaid; D C Wilton
Journal:  Biochem J       Date:  1995-06-01       Impact factor: 3.857
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