Literature DB >> 18981520

Polymerase chain reaction for chronic Trypanosoma cruzi infection yields higher sensitivity in blood clot than buffy coat or whole blood specimens.

Sean Fitzwater1, Maritza Calderon, Carlos Lafuente, Gerson Galdos-Cardenas, Lisbeth Ferrufino, Manuela Verastegui, Robert H Gilman, Caryn Bern.   

Abstract

Trypanosoma cruzi polymerase chain reaction (PCR) is widely used, but sensitivity varies widely. We compared PCR using 121/122 primers targeting kinetoplast minicircle DNA in whole blood, buffy coat, and clot from Bolivian women. Sensitivity was significantly higher in clot (60.1%) than buffy coat (46.5%) or whole blood (40%). The use of clot could simplify specimen collection while improving sensitivity.

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Year:  2008        PMID： 18981520

Source DB:  PubMed          Journal:  Am J Trop Med Hyg        ISSN： 0002-9637            Impact factor:   2.345


  20 in total

1.  Congenital Trypanosoma cruzi transmission in Santa Cruz, Bolivia.

Authors:  Caryn Bern; Manuela Verastegui; Robert H Gilman; Carlos Lafuente; Gerson Galdos-Cardenas; Maritza Calderon; Juan Pacori; Maria Del Carmen Abastoflor; Hugo Aparicio; Mark F Brady; Lisbeth Ferrufino; Noelia Angulo; Sarah Marcus; Charles Sterling; James H Maguire
Journal:  Clin Infect Dis       Date:  2009-12-01       Impact factor: 9.079

2.  Cavia porcellus as a model for experimental infection by Trypanosoma cruzi.

Authors:  Yagahira E Castro-Sesquen; Robert H Gilman; Verónica Yauri; Noelia Angulo; Manuela Verastegui; Daniel E Velásquez; Charles R Sterling; Diana Martin; Caryn Bern
Journal:  Am J Pathol       Date:  2011-05-14       Impact factor: 4.307

3.  Development of a Novel Protocol Based on Blood Clot to Improve the Sensitivity of qPCR Detection of Toxoplasma gondii in Peripheral Blood Specimens.

Authors:  Renzo Gutierrez-Loli; Cusi Ferradas; Andrea Diestra; Aliki Traianou; Natalie Bowman; Jeroen Bok; Melissa Reimer-McAtee; Cesar Ramal; Eduardo Ticona; Hannah Steinberg; Holger Mayta; Maritza Calderon; Jaeson S Calla-Choque; Charles Sterling; Robert H Gilman
Journal:  Am J Trop Med Hyg       Date:  2019-01       Impact factor: 2.345

4.  Chagas cardiomyopathy in the context of the chronic disease transition.

Authors:  Alicia I Hidron; Robert H Gilman; Juan Justiniano; Anna J Blackstock; Carlos Lafuente; Walter Selum; Martiza Calderon; Manuela Verastegui; Lisbeth Ferrufino; Eduardo Valencia; Jeffrey A Tornheim; Seth O'Neal; Robert Comer; Gerson Galdos-Cardenas; Caryn Bern
Journal:  PLoS Negl Trop Dis       Date:  2010-05-18

5.  Trypomastigote Excretory Secretory Antigen Blot Is Associated With Trypanosoma cruzi Load and Detects Congenital T. cruzi Infection in Neonates, Using Anti-Shed Acute Phase Antigen Immunoglobulin M.

Authors:  Sassan Noazin; Jessica A Lee; Edith S Malaga; Edward Valencia Ayala; Beth J Condori; Cristian Roca; Andres G Lescano; Caryn Bern; Walter Castillo; Holger Mayta; Maria Carmen Menduiña; Manuela R Verastegui; Freddy Tinajeros; Robert H Gilman
Journal:  J Infect Dis       Date:  2019-01-29       Impact factor: 5.226

6.  Domestic Pig (Sus scrofa) as an Animal Model for Experimental Trypanosoma cruzi Infection.

Authors:  Verónica Yauri; Yagahira E Castro-Sesquen; Manuela Verastegui; Noelia Angulo; Fernando Recuenco; Ines Cabello; Edith Malaga; Caryn Bern; Cesar M Gavidia; Robert H Gilman
Journal:  Am J Trop Med Hyg       Date:  2016-02-29       Impact factor: 2.345

Review 7.  ELISA versus PCR for diagnosis of chronic Chagas disease: systematic review and meta-analysis.

Authors:  Pedro E A A Brasil; Liane De Castro; Alejandro M Hasslocher-Moreno; Luiz H C Sangenis; José U Braga
Journal:  BMC Infect Dis       Date:  2010-11-25       Impact factor: 3.090

8.  Sensitive and specific detection of Trypanosoma cruzi DNA in clinical specimens using a multi-target real-time PCR approach.

Authors:  Yvonne Qvarnstrom; Alejandro G Schijman; Vincent Veron; Christine Aznar; Francis Steurer; Alexandre J da Silva
Journal:  PLoS Negl Trop Dis       Date:  2012-07-03

9.  Usefulness of real time PCR to quantify parasite load in serum samples from chronic Chagas disease patients.

Authors:  Myllena F Melo; Otacilio C Moreira; Priscila Tenório; Virginia Lorena; Izaura Lorena-Rezende; Wilson Oliveira Júnior; Yara Gomes; Constança Britto
Journal:  Parasit Vectors       Date:  2015-03-12       Impact factor: 3.876

10.  Detection of soluble antigen and DNA of Trypanosoma cruzi in urine is independent of renal injury in the guinea pig model.

Authors:  Yagahira E Castro-Sesquen; Robert H Gilman; Verónica Yauri; Jaime Cok; Noelia Angulo; Hermes Escalante; Caryn Bern
Journal:  PLoS One       Date:  2013-03-08       Impact factor: 3.240
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