Literature DB >> 1898022

Purification and immunocharacterization of a plant cytochrome P450: the cinnamic acid 4-hydroxylase.

B Gabriac1, D Werck-Reichhart, H Teutsch, F Durst.   

Abstract

Cinnamic acid 4-hydroxylase (CA4H) was purified from microsomes of manganese-induced Jerusalem artichoke (Helianthus tuberosus L.) tuber tissues. The three-step purification procedure involved solubilization and phase partitioning in Triton X-114, followed by chromatography on DEAE-Trisacryl and hydroxylapatite columns. Purification was monitored using carbon monoxide and type I substrate binding properties of the enzyme. The protein, purified to electrophoretic homogeneity, showed an Mr of about 57,000 on SDS-PAGE. Polyclonal antibodies raised against this protein selectively reacted with a 57-kDa polypeptide on Western blots of induced Jerusalem artichoke microsomes. The antibody selectively and strongly inhibited CA4H activity from several plant species.

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Year:  1991        PMID： 1898022     DOI： 10.1016/0003-9861(91)90199-s

Source DB:  PubMed          Journal:  Arch Biochem Biophys        ISSN： 0003-9861            Impact factor:   4.013


  15 in total

1.  Isolation and characterization of a gene encoding cinnamate 4-hydroxylase from Parthenocissus henryana.

Authors:  Shujun Liu; Yuanlei Hu; Xiaoli Wang; Li Han; Songquan Song; Hongyan Cheng; Zhongping Lin
Journal:  Mol Biol Rep       Date:  2008-09-13       Impact factor: 2.316

2.  Cytochrome P450 monooxygenases for fatty acids and xenobiotics in marine macroalgae.

Authors:  S Pflugmacher; H Sandermann
Journal:  Plant Physiol       Date:  1998-05       Impact factor: 8.340

3.  RNAi suppression of Arogenate Dehydratase1 reveals that phenylalanine is synthesized predominantly via the arogenate pathway in petunia petals.

Authors:  Hiroshi Maeda; Ajit K Shasany; Jennifer Schnepp; Irina Orlova; Goro Taguchi; Bruce R Cooper; David Rhodes; Eran Pichersky; Natalia Dudareva
Journal:  Plant Cell       Date:  2010-03-09       Impact factor: 11.277

4.  Induction of Benzoic Acid 2-Hydroxylase in Virus-Inoculated Tobacco.

Authors:  J. Leon; N. Yalpani; I. Raskin; M. A. Lawton
Journal:  Plant Physiol       Date:  1993-10       Impact factor: 8.340

5.  Expression of a Ripening-Related Avocado (Persea americana) Cytochrome P450 in Yeast.

Authors:  K R Bozak; D P O'keefe; R E Christoffersen
Journal:  Plant Physiol       Date:  1992-12       Impact factor: 8.340

6.  Molecular Analysis and Heterologous Expression of an Inducible Cytochrome P-450 Protein from Periwinkle (Catharanthus roseus L.).

Authors:  H P Vetter; U Mangold; G Schröder; F J Marner; D Werck-Reichhart; J Schröder
Journal:  Plant Physiol       Date:  1992-10       Impact factor: 8.340

7.  Regulation and functional expression of cinnamate 4-hydroxylase from parsley.

Authors:  E Koopmann; E Logemann; K Hahlbrock
Journal:  Plant Physiol       Date:  1999-01       Impact factor: 8.340

8.  Isolation and sequence of a cDNA encoding the Jerusalem artichoke cinnamate 4-hydroxylase, a major plant cytochrome P450 involved in the general phenylpropanoid pathway.

Authors:  H G Teutsch; M P Hasenfratz; A Lesot; C Stoltz; J M Garnier; J M Jeltsch; F Durst; D Werck-Reichhart
Journal:  Proc Natl Acad Sci U S A       Date:  1993-05-01       Impact factor: 11.205

9.  Engineering of a water-soluble plant cytochrome P450, CYP73A1, and NMR-based orientation of natural and alternate substrates in the active site.

Authors:  Guillaume A Schoch; Roger Attias; Maya Belghazi; Patrick M Dansette; Danièle Werck-Reichhart
Journal:  Plant Physiol       Date:  2003-10-23       Impact factor: 8.340

10.  The chemically inducible plant cytochrome P450 CYP76B1 actively metabolizes phenylureas and other xenobiotics

Authors: 
Journal:  Plant Physiol       Date:  1998-11       Impact factor: 8.340
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