| Literature DB >> 18979172 |
Tatiana Salles de Souza Malaspina1, Célio Xavier dos Santos, Ana Paula Campanelli, Francisco Rafael Martins Laurindo, Mari Cleide Sogayar, José Mauro Granjeiro.
Abstract
Tartrate-resistant acid phosphatase (TRAP) is a well-known marker of osteoclasts and bone resorption. Here we have investigated whether osteoblast-like cells (hFOB 1.19) present TRAP activity and how would be its pattern of expression during osteoblastic differentiation. We also observed how the osteoblastic differentiation affected the reduced glutathione levels. TRAP activity was measured using the p-nitrophenylphosphate substrate. The osteogenic potential of hFOB 1.19 cells was studied by measuring alkaline phosphatase activity and mineralized nodule formation. Oxidative stress was determined by HPLC and DNTB assays. TRAP activity and the reduced glutathione-dependent microenvironment were modulated during osteoblastic differentiation. During this phase, TRAP activity, as well as alkaline phosphatase and glutathione increased progressively up to the 21st day, decreasing thereafter. We demonstrate that TRAP activity is modulated during osteoblastic differentiation, possibly in response to the redox state of the cell, since it seemed to depend on suitable levels of reduced glutathione.Entities:
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Year: 2008 PMID： 18979172 DOI： 10.1007/s10735-008-9203-0
Source DB: PubMed Journal: J Mol Histol ISSN： 1567-2379 Impact factor: 2.611