BACKGROUND: Although fasting and post-methionine loading (PML) homocysteine concentrations are not necessarily related, a high percentage of hyperhomocysteinemia cases would be missed if methionine loading was not performed. AIM OF THE STUDY: The influences of B-vitamins and genetic polymorphism (methylenetetrahydrofolate reductase 677C --> T, MTHFR 677C --> T) on fasting and PML homocysteine concentrations and the relationship between fasting and PML homocysteine were studied. METHODS: This study was a cross-sectional study. Healthy subjects were divided into either fasting hyper-homocysteinemia (>or=12.2 micromol/l) (fasting hyper-hcy, n = 51), PML hyper-homocysteinemia (fasting homocysteine <12.2 micromol/l but PML homocysteine >or=25.6 micromol/l) (PML hyper-hcy, n = 29), or normo-homocysteinemia (fasting homocysteine <12.2 micromol/l and PML homocysteine <25.6 micromol/l) (normo-hcy, n = 118) group based on elevated fasting and PML homocysteine levels of the 75th percentile of the population. The concentrations of plasma fasting and PML homocysteine, serum folate, vitamin B-12, plasma pyridoxal 5'- phosphate (PLP) were measured. The genetic polymorphisms were determined. RESULTS: Fasting homocysteine, but not PML homocysteine and MTHFR 677C --> T genotype, was significantly and inversely affected by serum folate concentration after adjusting for potential confounders (beta = -0.062, P < 0.01). Fasting and PML homocysteine were highly associated in the fasting hyper-hcy and pooled groups (P < 0.01) but not in the PML hyper-hcy and normo-hcy groups. PML homocysteine did not interact with either serum folate (P = 0.302), vitamin B-12 (P = 0.465), plasma PLP (P = 0.996) or MTHFR 677C --> T genotype (P = 0.136) to affect fasting homocysteine concentration. CONCLUSIONS: Approximately one-third (36.3%) of hyperhomocysteinemia cases would be missed if methionine loading were not performed. Even though subjects may have a normal fasting homocysteine concentration, they need further screening for their PML homocysteine.
BACKGROUND: Although fasting and post-methionine loading (PML) homocysteine concentrations are not necessarily related, a high percentage of hyperhomocysteinemia cases would be missed if methionine loading was not performed. AIM OF THE STUDY: The influences of B-vitamins and genetic polymorphism (methylenetetrahydrofolate reductase 677C --> T, MTHFR 677C --> T) on fasting and PML homocysteine concentrations and the relationship between fasting and PML homocysteine were studied. METHODS: This study was a cross-sectional study. Healthy subjects were divided into either fasting hyper-homocysteinemia (>or=12.2 micromol/l) (fasting hyper-hcy, n = 51), PML hyper-homocysteinemia (fasting homocysteine <12.2 micromol/l but PML homocysteine >or=25.6 micromol/l) (PML hyper-hcy, n = 29), or normo-homocysteinemia (fasting homocysteine <12.2 micromol/l and PML homocysteine <25.6 micromol/l) (normo-hcy, n = 118) group based on elevated fasting and PML homocysteine levels of the 75th percentile of the population. The concentrations of plasma fasting and PML homocysteine, serum folate, vitamin B-12, plasma pyridoxal 5'- phosphate (PLP) were measured. The genetic polymorphisms were determined. RESULTS: Fasting homocysteine, but not PML homocysteine and MTHFR 677C --> T genotype, was significantly and inversely affected by serum folate concentration after adjusting for potential confounders (beta = -0.062, P < 0.01). Fasting and PML homocysteine were highly associated in the fasting hyper-hcy and pooled groups (P < 0.01) but not in the PML hyper-hcy and normo-hcy groups. PML homocysteine did not interact with either serum folate (P = 0.302), vitamin B-12 (P = 0.465), plasma PLP (P = 0.996) or MTHFR 677C --> T genotype (P = 0.136) to affect fasting homocysteine concentration. CONCLUSIONS: Approximately one-third (36.3%) of hyperhomocysteinemia cases would be missed if methionine loading were not performed. Even though subjects may have a normal fasting homocysteine concentration, they need further screening for their PML homocysteine.
Authors: M van den Berg; S C de Jong; W Devillé; J A Rauwerda; C Jakobs; G Pals; G H Boers; C D Stehouwer Journal: Neth J Med Date: 1999-07 Impact factor: 1.422
Authors: L Brattström; B Israelsson; B Norrving; D Bergqvist; J Thörne; B Hultberg; A Hamfelt Journal: Atherosclerosis Date: 1990-02 Impact factor: 5.162
Authors: Dattatray S Bhat; Lourdes L Gruca; Carole D Bennett; Prachi Katre; Anura V Kurpad; Chittaranjan S Yajnik; Satish C Kalhan Journal: PLoS One Date: 2018-05-24 Impact factor: 3.240
Authors: May A Beydoun; Hind A Beydoun; Peter H MacIver; Sharmin Hossain; Jose A Canas; Michele K Evans; Alan B Zonderman Journal: Nutrients Date: 2020-03-30 Impact factor: 5.717