Literature DB >> 18979027

Protocol for RNAi assays in adult mosquitoes (A. gambiae).

Lindsey Garver1, George Dimopoulos.   

Abstract

Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the Dimopoulos lab to inject dsRNA into Anopheles gambiae mosquitoes, which harbor the malaria parasite. The technique manipulating the injection setup and injecting dsRNA into the thorax is illustrated.

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Year:  2007        PMID： 18979027      PMCID： PMC2557093          DOI： 10.3791/230

Source DB:  PubMed          Journal:  J Vis Exp        ISSN： 1940-087X            Impact factor:   1.355


  16 in total

1.  RNAi-mediated gene knockdown and in vivo diuresis assay in adult female Aedes aegypti mosquitoes.

Authors:  Lisa L Drake; David P Price; Sarah E Aguirre; Immo A Hansen
Journal:  J Vis Exp       Date:  2012-07-14       Impact factor: 1.355

2.  Dengue virus infection of the Aedes aegypti salivary gland and chemosensory apparatus induces genes that modulate infection and blood-feeding behavior.

Authors:  Shuzhen Sim; José L Ramirez; George Dimopoulos
Journal:  PLoS Pathog       Date:  2012-03-29       Impact factor: 6.823

3.  Wolbachia induces reactive oxygen species (ROS)-dependent activation of the Toll pathway to control dengue virus in the mosquito Aedes aegypti.

Authors:  Xiaoling Pan; Guoli Zhou; Jiahong Wu; Guowu Bian; Peng Lu; Alexander S Raikhel; Zhiyong Xi
Journal:  Proc Natl Acad Sci U S A       Date:  2011-11-28       Impact factor: 11.205

4.  Innexin AGAP001476 is critical for mediating anti-Plasmodium responses in Anopheles mosquitoes.

Authors:  Michelle W M Li; Jiuling Wang; Yang O Zhao; Erol Fikrig
Journal:  J Biol Chem       Date:  2014-07-17       Impact factor: 5.157

5.  Aedes aegypti ML and Niemann-Pick type C family members are agonists of dengue virus infection.

Authors:  Natapong Jupatanakul; Shuzhen Sim; George Dimopoulos
Journal:  Dev Comp Immunol       Date:  2013-10-14       Impact factor: 3.636

6.  Role of G-protein-coupled receptor-related genes in insecticide resistance of the mosquito, Culex quinquefasciatus.

Authors:  Ting Li; Lena Liu; Lee Zhang; Nannan Liu
Journal:  Sci Rep       Date:  2014-09-29       Impact factor: 4.379

7.  Production of Infectious Dengue Virus in Aedes aegypti Is Dependent on the Ubiquitin Proteasome Pathway.

Authors:  Milly M Choy; October M Sessions; Duane J Gubler; Eng Eong Ooi
Journal:  PLoS Negl Trop Dis       Date:  2015-11-13

8.  Transcriptomic profiling of diverse Aedes aegypti strains reveals increased basal-level immune activation in dengue virus-refractory populations and identifies novel virus-vector molecular interactions.

Authors:  Shuzhen Sim; Natapong Jupatanakul; José L Ramirez; Seokyoung Kang; Claudia M Romero-Vivas; Hamish Mohammed; George Dimopoulos
Journal:  PLoS Negl Trop Dis       Date:  2013-07-04

9.  FlyNap (triethylamine) increases the heart rate of mosquitoes and eliminates the cardioacceleratory effect of the neuropeptide CCAP.

Authors:  Weihan Chen; Julián F Hillyer
Journal:  PLoS One       Date:  2013-07-16       Impact factor: 3.240

10.  Circadian clock of Aedes aegypti: effects of blood-feeding, insemination and RNA interference.

Authors:  Carla Gentile; Gustavo Bueno da S Rivas; José B P Lima; Rafaela Vieira Bruno; Alexandre Afranio Peixoto
Journal:  Mem Inst Oswaldo Cruz       Date:  2013       Impact factor: 2.743
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