Literature DB >> 18979015

Injection of dsRNA into female A. aegypti mosquitos.

Brian M Luna1, Jennifer Juhn, Anthony A A James.   

Abstract

Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.

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Year:  2007        PMID： 18979015      PMCID： PMC2557097          DOI： 10.3791/215

Source DB:  PubMed          Journal:  J Vis Exp        ISSN： 1940-087X            Impact factor:   1.355


  7 in total

1.  Aedes aegypti: an emerging model for vector mosquito development.

Authors:  Anthony Clemons; Morgan Haugen; Ellen Flannery; Michael Tomchaney; Kristopher Kast; Caitlin Jacowski; Christy Le; Akio Mori; Wendy Simanton Holland; Joseph Sarro; David W Severson; Molly Duman-Scheel
Journal:  Cold Spring Harb Protoc       Date:  2010-10-01

2.  Functional analysis of genes in Aedes aegypti embryos.

Authors:  Anthony Clemons; Morgan Haugen; David Severson; Molly Duman-Scheel
Journal:  Cold Spring Harb Protoc       Date:  2010-10-01

3.  RNAi-mediated double gene knockdown and gustatory perception measurement in honey bees (Apis mellifera).

Authors:  Ying Wang; Nicholas Baker; Gro V Amdam
Journal:  J Vis Exp       Date:  2013-07-25       Impact factor: 1.355

4.  Molecular basis of the remarkable species selectivity of an insecticidal sodium channel toxin from the African spider Augacephalus ezendami.

Authors:  Volker Herzig; Maria Ikonomopoulou; Jennifer J Smith; Sławomir Dziemborowicz; John Gilchrist; Lucia Kuhn-Nentwig; Fernanda Oliveira Rezende; Luciano Andrade Moreira; Graham M Nicholson; Frank Bosmans; Glenn F King
Journal:  Sci Rep       Date:  2016-07-07       Impact factor: 4.379

5.  Functional analysis of a novel parasitic nematode-specific protein of Setaria digitata larvae in Culex quinquefasciatus by siRNA mediated RNA interference.

Authors:  Magalla Bastian Chalitha Lakmal Somarathne; Yasanthi Illika Nilmini Silva Gunawardene; Naduvilath Vishvanath Chandrasekharan; Arjuna Nisantha Bandara Ellepola; Ranil Samantha Dassanayake
Journal:  Parasit Vectors       Date:  2018-10-10       Impact factor: 3.876

6.  Improvised microinjection technique for mosquito vectors.

Authors:  S Sampath Kumar; H P Puttaraju
Journal:  Indian J Med Res       Date:  2012-12       Impact factor: 2.375

7.  Functional characterization of three MicroRNAs of the Asian tiger mosquito, Aedes albopictus.

Authors:  Santhosh Puthiyakunnon; Yunying Yao; Yiji Li; Jinbao Gu; Hongjuan Peng; Xiaoguang Chen
Journal:  Parasit Vectors       Date:  2013-08-08       Impact factor: 3.876
  7 in total

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