BACKGROUND: The morphology of the invasive margin in colorectal cancer can be described as either pushing or infiltrative. These phenotypes carry prognostic significance, particularly in node negative disease, and provide an excellent model for the study of invasive behaviour in vivo. METHODS: The marginal edges of 16 stage-matched tumours exhibiting these contrasting growth patterns were microdissected. The extracted mRNA was amplified and hybridised to a 9546 feature oligonucleotide array. Selected differentials were validated using real-time polymerase chain reaction and the protein product was interrogated by using immunohistochemistry. RESULTS: After stringent quality control and filtering of data generated, 39 genes were identified as being significantly differentially expressed between the two types of marginal edge. Several genes involved in cellular metabolism were identified as differentials including lactate dehydrogenase B (LDHB) and modulators of glucose transport. CONCLUSIONS: The LDH expression profile differs between the invasive phenotypes. A hypothesis is proposed in which altered metabolism is a cause of contrasting invasive behaviour independent of the hypoxia-inducible factor mediated hypoxic response, consistent with the Warburg phenomenon.
BACKGROUND: The morphology of the invasive margin in colorectal cancer can be described as either pushing or infiltrative. These phenotypes carry prognostic significance, particularly in node negative disease, and provide an excellent model for the study of invasive behaviour in vivo. METHODS: The marginal edges of 16 stage-matched tumours exhibiting these contrasting growth patterns were microdissected. The extracted mRNA was amplified and hybridised to a 9546 feature oligonucleotide array. Selected differentials were validated using real-time polymerase chain reaction and the protein product was interrogated by using immunohistochemistry. RESULTS: After stringent quality control and filtering of data generated, 39 genes were identified as being significantly differentially expressed between the two types of marginal edge. Several genes involved in cellular metabolism were identified as differentials including lactate dehydrogenase B (LDHB) and modulators of glucose transport. CONCLUSIONS: The LDH expression profile differs between the invasive phenotypes. A hypothesis is proposed in which altered metabolism is a cause of contrasting invasive behaviour independent of the hypoxia-inducible factor mediated hypoxic response, consistent with the Warburg phenomenon.
Authors: Anuli Uzozie; Paolo Nanni; Teresa Staiano; Jonas Grossmann; Simon Barkow-Oesterreicher; Jerry W Shay; Amit Tiwari; Federico Buffoli; Endre Laczko; Giancarlo Marra Journal: Mol Cell Proteomics Date: 2014-02-24 Impact factor: 5.911
Authors: Ingrid Moen; Anne Margrete Øyan; Karl-Henning Kalland; Karl Johan Tronstad; Lars Andreas Akslen; Martha Chekenya; Per Øystein Sakariassen; Rolf Kåre Reed; Linda Elin Birkhaug Stuhr Journal: PLoS One Date: 2009-07-28 Impact factor: 3.240
Authors: Stephanie M J Fliedner; Nina Kaludercic; Xiao-Sheng Jiang; Hana Hansikova; Zuzana Hajkova; Jana Sladkova; Andrea Limpuangthip; Peter S Backlund; Robert Wesley; Lucia Martiniova; Ivana Jochmanova; Nikoletta K Lendvai; Jan Breza; Alfred L Yergey; Nazareno Paolocci; Arthur S Tischler; Jiri Zeman; Forbes D Porter; Hendrik Lehnert; Karel Pacak Journal: PLoS One Date: 2012-07-31 Impact factor: 3.240