Suw Young Ly1, Nam Sun Cho. 1. Biosensor Research Institute, Seoul National University of Technology, 172, Gongreung 2-dong, Nowon-gu, Seoul 139-743, South Korea. suwyoung@snut.ac.kr
Abstract
OBJECTIVES: To identify an effective bioassay for human hepatitis B virus (HBV) using voltammetric sensor. STUDY DESIGN: Electrode was prepared with bovine IgG immobilized onto a DNA-linked carbon nanotube electrode (BIDCE). Analytical BIDCE conditions were optimized through square wave (SW) stripping and cyclic voltammetry (CV). RESULTS: Optimum parameters of 75 Hz SW frequency, 0.1 V SW amplitude, -1.3 V SW accumulation potential, 100 s accumulation time, and 3.0 mV SW increment potential were obtained. Within working ranges of 0.035-0.242 mg/mL anti-bovine IgG, the relative standard deviation of 0.2 mL HBV was observed to be 0.04 (n=4). CONCLUSIONS: Diagnostic application was performed through direct assay of HBV in non-treated human blood.
OBJECTIVES: To identify an effective bioassay for human hepatitis B virus (HBV) using voltammetric sensor. STUDY DESIGN: Electrode was prepared with bovine IgG immobilized onto a DNA-linked carbon nanotube electrode (BIDCE). Analytical BIDCE conditions were optimized through square wave (SW) stripping and cyclic voltammetry (CV). RESULTS: Optimum parameters of 75 Hz SW frequency, 0.1 V SW amplitude, -1.3 V SW accumulation potential, 100 s accumulation time, and 3.0 mV SW increment potential were obtained. Within working ranges of 0.035-0.242 mg/mL anti-bovine IgG, the relative standard deviation of 0.2 mL HBV was observed to be 0.04 (n=4). CONCLUSIONS: Diagnostic application was performed through direct assay of HBV in non-treated human blood.