OBJECTIVE: To explore the contraceptive potential of the CatSper1 transmembrane domains and pore region in vitro. DESIGN: In vitro study with human sperm and mouse fertilization. SETTING: Andrology laboratory of an academic research center. PATIENT(S) AND ANIMAL(S): Normozoospermia and viripotent BALB/c mice. INTERVENTION(S): The specific binding of an anti-CatSper1 IgG antibody (H-300) to CatSper1 was confirmed by Western blot and immunofluorescence. Sperm from humans and mice were incubated with H-300. MAIN OUTCOME MEASURE(S): The effects of H-300 on human sperm progressive motility, abnormal acrosome, hyperactivated motility, and mouse in vitro fertilization rates were analyzed. RESULT(S): A significant decline in sperm progressive motility was observed after 1, 2, and 4 hours of incubation with H-300; the change was mainly ascribed to the decline of fast progressive motility. Significant inhibition of the hyperactivated motility was observed after 5 hours of incubation with H-300. The incubation of mouse sperm with H-300 before insemination reduced the in vitro fertilization rate to 28% of control levels (72% inhibition). CONCLUSION(S): CatSper1 may be a potential target for immunocontraception, and the antibody may be a tool to study the function of ion channels in sperm in which relatively fewer methods can be applied.
OBJECTIVE: To explore the contraceptive potential of the CatSper1 transmembrane domains and pore region in vitro. DESIGN: In vitro study with human sperm and mouse fertilization. SETTING: Andrology laboratory of an academic research center. PATIENT(S) AND ANIMAL(S): Normozoospermia and viripotent BALB/c mice. INTERVENTION(S): The specific binding of an anti-CatSper1 IgG antibody (H-300) to CatSper1 was confirmed by Western blot and immunofluorescence. Sperm from humans and mice were incubated with H-300. MAIN OUTCOME MEASURE(S): The effects of H-300 on human sperm progressive motility, abnormal acrosome, hyperactivated motility, and mouse in vitro fertilization rates were analyzed. RESULT(S): A significant decline in sperm progressive motility was observed after 1, 2, and 4 hours of incubation with H-300; the change was mainly ascribed to the decline of fast progressive motility. Significant inhibition of the hyperactivated motility was observed after 5 hours of incubation with H-300. The incubation of mouse sperm with H-300 before insemination reduced the in vitro fertilization rate to 28% of control levels (72% inhibition). CONCLUSION(S): CatSper1 may be a potential target for immunocontraception, and the antibody may be a tool to study the function of ion channels in sperm in which relatively fewer methods can be applied.
Authors: Michael S Hildebrand; Matthew R Avenarius; Marc Fellous; Yuzhou Zhang; Nicole C Meyer; Jana Auer; Catherine Serres; Kimia Kahrizi; Hossein Najmabadi; Jacques S Beckmann; Richard J H Smith Journal: Eur J Hum Genet Date: 2010-07-21 Impact factor: 4.246
Authors: Matthew R Avenarius; Michael S Hildebrand; Yuzhou Zhang; Nicole C Meyer; Luke L H Smith; Kimia Kahrizi; Hossein Najmabadi; Richard J H Smith Journal: Am J Hum Genet Date: 2009-04-02 Impact factor: 11.025