Ye Tian1, Zhige Shi, Shu Yang, Yingzhu Chen, Shiyao Bao. 1. Department of Radiotherapy and Oncology, Second Affiliated Hospital, Suzhou University, Jiangsu, People's Republic of China. dryetian@hotmail.com
Abstract
OBJECT: The aim of this study was to determine the relation between changes in myelin basic protein (MBP) levels during the acute and subacute phases of central nervous system injury following whole-brain radiation and delayed demyelination in the radiation-injured brain tissue. METHODS: Adult Sprague-Dawley rats were treated with single fractions of 2, 10, or 30 Gy of whole-brain radiation. The authors measured MBP gene expression and protein levels in the brain tissue by using reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay at 1 week and 1-3 months following irradiation to monitor myelin changes in the brain. Demyelination was determined with Luxol fast blue myelin staining and routine histopathological and electron microscopy examination of injured brain tissue. The changes in MBP levels in the different animal groups at specific time points were correlated with demyelination in corresponding dose groups. RESULTS: At 1 month after applying the 10 and 30 Gy of radiation, MBP mRNA expression showed a transient but significant decrease, followed by recovery to baseline levels at 3 months after treatment. The MBP levels were decreased by only 70-75% at 1 month after 10 and 30 Gy of radiation. At 2-3 months after applying the higher dose of 30 Gy, however, the MBP levels continued to decline, and typical demyelination changes were observed with myelin staining and ultrastructural examination. CONCLUSIONS: The authors' results suggest that the early radiation-induced MBP changes between 1 and 3 months after single treatments of 10 and 30 Gy of radiation to the whole brain are indicative of permanent injury shown as demyelination of irradiated brain tissue.
OBJECT: The aim of this study was to determine the relation between changes in myelin basic protein (MBP) levels during the acute and subacute phases of central nervous system injury following whole-brain radiation and delayed demyelination in the radiation-injured brain tissue. METHODS: Adult Sprague-Dawley rats were treated with single fractions of 2, 10, or 30 Gy of whole-brain radiation. The authors measured MBP gene expression and protein levels in the brain tissue by using reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay at 1 week and 1-3 months following irradiation to monitor myelin changes in the brain. Demyelination was determined with Luxol fast blue myelin staining and routine histopathological and electron microscopy examination of injured brain tissue. The changes in MBP levels in the different animal groups at specific time points were correlated with demyelination in corresponding dose groups. RESULTS: At 1 month after applying the 10 and 30 Gy of radiation, MBP mRNA expression showed a transient but significant decrease, followed by recovery to baseline levels at 3 months after treatment. The MBP levels were decreased by only 70-75% at 1 month after 10 and 30 Gy of radiation. At 2-3 months after applying the higher dose of 30 Gy, however, the MBP levels continued to decline, and typical demyelination changes were observed with myelin staining and ultrastructural examination. CONCLUSIONS: The authors' results suggest that the early radiation-induced MBP changes between 1 and 3 months after single treatments of 10 and 30 Gy of radiation to the whole brain are indicative of permanent injury shown as demyelination of irradiated brain tissue.
Authors: Janice A Zawaski; M Waleed Gaber; Omaima M Sabek; Christy M Wilson; Christopher D Duntsch; Thomas E Merchant Journal: Int J Radiat Oncol Biol Phys Date: 2011-12-22 Impact factor: 7.038
Authors: Raphaël Serduc; Audrey Bouchet; Benoît Pouyatos; Luc Renaud; Elke Bräuer-Krisch; Géraldine Le Duc; Jean A Laissue; Stefan Bartzsch; Nicolas Coquery; Yohan van de Looij Journal: PLoS One Date: 2014-02-05 Impact factor: 3.240