Peroxidase immobilized on Amberlite IRA-743 resin for on-line spectrophotometric detection of hydrogen peroxide in rainwater.

The importance of atmospheric hydrogen peroxide (H(2)O(2)) in the oxidation of SO(2) and other compounds has been well established. A spectrophotometric method for the determination of hydrogen peroxide in rainwater is proposed. This method is based on selective oxidation of hydrogen peroxide using an on-line tubular reactor containing peroxidase immobilized on Amberlite IRA-743 resin. The hydrogen peroxide in the presence of phenol, 4-aminoantipyrine and peroxidase, produces a red compound (lambda=505 nm). Beer's law is obeyed in a concentration range of 1-100 microl l(-1) hydrogen peroxide with an excellent correlation coefficient (r=0.9991), at pH 7.0, with a relative standard deviation (R.S.D.) <2%. The detection limit of the method is 0.7 micromol l(-1) (4.8 ng of H(2)O(2) in a 200 microl sample). Measurements of hydrogen peroxide in rain samples were carried out over the period from November 2003 to January 2005, in the central area of the Juiz de Fora city, Brazil. The concentration of H(2)O(2) varied from values lower than the detection limit to 92.5 microl l(-1). The effects of the presence of non-sea salt (NSS) SO(4)(2-), NO(3)(-) and H(+) in the concentration of hydrogen peroxide in the rainwater had been evaluated. The average concentrations of H(2)O(2), NO(3)(-), NSS SO(4)(2-) and SO(4)(2-) are 23.4, 18.9, 7.9 and 10.3 microl l(-1), respectively. The pH values for 82% of the collected samples are greater than 5.0. The spectrophotometeric method developed in this work that uses enzyme immobilized on the resin ion-exchange compared with the amperometric method did not present any significant difference in the results.