Flow-injection analysis of two fluoquinolones by the sensitizing effect of terbium(III) on chemiluminescence of the potassium permanganate-sodium sulfite system.

A simple, rapid and sensitive method for the determination of two fluoquinolones (FQs), enoxacin (ENX) and ofloxacin (OFLX) is described by using flow injection analysis with potassium permanganate-sodium sulfite chemiluminescence detection. The calibration graphs for ENX and OFLX are linear in the range of 8.0x10(-10)-1.0x10(-5) and 1.0x10(-9)-1.0x10(-6) mol l(-1), respectively. The 3sigma limits of detection are 2.4x10(-10) mol l(-1) for ENX and 5.6x10(-10) mol l(-1) for OFLX. The method is applied satisfactorily to the determination of the two FQs in dosage form and urine sample. The possible mechanism is also proposed.