Simultaneous determination of propranolol and pindolol by synchronous spectrofluorimetry.

The simultaneous determination of propranolol and pindolol using synchronous fluorescence spectrometric techniques is described. The method involves measuring the natural fluorescence of these drugs in a 50% (v/v) ethanol-water medium using zero and first derivative synchronous spectrofluorimetry. Under the optimum conditions, the linear determination ranges of propanolol and pindolol are ca. 0.02-1.0 and 0.04-1.2 mug ml(-1), respectively. The results showed that propranolol and pindolol can be determined simultaneously when the concentration ratio of propranolol to pindolol varies from 1:100 to 50:1 in the mixed sample. The method has been satisfactorily applied to the determination of propranolol and pindolol in urine samples and propranolol in pharmaceutical preparations.