Simultaneous determination of warfarin and bromadiolone by derivative synchronous fluorescence spectrometry.

By using second derivative synchronous fluorescence spectrometry the simple resolution of mixtures of the anticoagulant rodenticides warfarin and bromadiolone in the presence of beta-cyclodextrin is accomplished which causes a differential effect on the fluorescence intensity of these compounds. The determination method developed is simple, fast and inexpensive; in addition, measurements are performed in a single scan. Mixtures of warfarin and bromadiolone in ratios between 4:1 and 1:10 were satisfactorily resolved.