OBJECTIVES: The aim of this study was to determine the prevalence and characteristics of qnr-carrying Salmonella isolates from humans in southern Taiwan. METHODS: A total of 446 Salmonella isolates collected between 2003 and 2006 were screened for qnrA, qnrB and qnrS by PCR experiments. Genetic structures of qnr were determined by PCR-based methods or direct sequencing of plasmid DNA. RESULTS: qnrB2 and qnrS1 were detected in two serovar Enteritidis isolates and two serovar Typhimurium isolates, respectively. One qnrS1-positive isolate was found to produce the CMY-2 AmpC enzyme. qnrS1 was identified on a 10 kb plasmid, which exhibited >99% nucleotide sequence identity with plasmid TPqnrS-1a reported from the UK. qnrB2 was found in a complex sul1-type class 1 integron on a >100 kb plasmid. CONCLUSIONS: This study demonstrated the occurrence of qnrB2 and qnrS1 in Salmonella for the first time in Taiwan and characterized their genetic structures.
OBJECTIVES: The aim of this study was to determine the prevalence and characteristics of qnr-carrying Salmonella isolates from humans in southern Taiwan. METHODS: A total of 446 Salmonella isolates collected between 2003 and 2006 were screened for qnrA, qnrB and qnrS by PCR experiments. Genetic structures of qnr were determined by PCR-based methods or direct sequencing of plasmid DNA. RESULTS: qnrB2 and qnrS1 were detected in two serovar Enteritidis isolates and two serovar Typhimurium isolates, respectively. One qnrS1-positive isolate was found to produce the CMY-2 AmpC enzyme. qnrS1 was identified on a 10 kb plasmid, which exhibited >99% nucleotide sequence identity with plasmid TPqnrS-1a reported from the UK. qnrB2 was found in a complex sul1-type class 1 integron on a >100 kb plasmid. CONCLUSIONS: This study demonstrated the occurrence of qnrB2 and qnrS1 in Salmonella for the first time in Taiwan and characterized their genetic structures.
Authors: Chin-Yi Chen; Rebecca L Lindsey; Terence P Strobaugh; Jonathan G Frye; Richard J Meinersmann Journal: Appl Environ Microbiol Date: 2010-08-06 Impact factor: 4.792
Authors: Vien Le; Nguyen Thi Khanh Nhu; Ana Cerdeno-Tarraga; James I Campbell; Ha Thanh Tuyen; Tran Do Hoang Nhu; Pham Thi Thanh Tam; Constance Schultsz; Guy Thwaites; Nicholas R Thomson; Stephen Baker Journal: J Med Microbiol Date: 2015-08 Impact factor: 2.472
Authors: Chin-Yi Chen; Terence P Strobaugh; Ly-Huong T Nguyen; Melanie Abley; Rebecca L Lindsey; Charlene R Jackson Journal: PLoS One Date: 2018-03-07 Impact factor: 3.240