OBJECTIVE: To investigate the association of IL-10 gene promoter polymorphism with susceptibility to hepatitis B viral infection in Han, Yi and Yao ethnic groups from Guizhou province. METHODS: Five hundred volunteers from Guizhou province were selected to undertake PCR-RFLP for detection of IL-10 gene promoter -592 polymorphism. RESULTS: The genotypic distributions of IL-10-592 were 32.53%-51.43% (AA), 40.74%-54.82% (AC), 5.79%-18.52% (CC) whereas the allelic frequencies were 59.94%-72.38% for the A allele, and 27.62%-40.06% for the C allele in Han, Yi and Yao ethnic from Guizhou. The distributions of allele and genotype frequencies of IL-10-592 were statistically different between Yao ethnic in Libo and Yi ethnic in Qianxi, Yao ethnic in Libo and Han ethnic in Libo,Yi ethnic in Qianxi and Yi ethnic in Weining,Yi ethnic in Weining and Han ethnic in Libo (P < 0.05). IL-10-592 polymorphism was associated with HBV infection in Yi ethnic in Qianxi and the whole population. CONCLUSION: IL-10-592 gene polymorphisms influenced the susceptibility to HBV infection in Han, Yao, Yi sub-populations in Guizhou. Result of the study suggested that IL-10-592 gene polymorphisms might serve as a risk factor to HBV infection.
OBJECTIVE: To investigate the association of IL-10 gene promoter polymorphism with susceptibility to hepatitis B viral infection in Han, Yi and Yao ethnic groups from Guizhou province. METHODS: Five hundred volunteers from Guizhou province were selected to undertake PCR-RFLP for detection of IL-10 gene promoter -592 polymorphism. RESULTS: The genotypic distributions of IL-10-592 were 32.53%-51.43% (AA), 40.74%-54.82% (AC), 5.79%-18.52% (CC) whereas the allelic frequencies were 59.94%-72.38% for the A allele, and 27.62%-40.06% for the C allele in Han, Yi and Yao ethnic from Guizhou. The distributions of allele and genotype frequencies of IL-10-592 were statistically different between Yao ethnic in Libo and Yi ethnic in Qianxi, Yao ethnic in Libo and Han ethnic in Libo,Yi ethnic in Qianxi and Yi ethnic in Weining,Yi ethnic in Weining and Han ethnic in Libo (P < 0.05). IL-10-592 polymorphism was associated with HBV infection in Yi ethnic in Qianxi and the whole population. CONCLUSION:IL-10-592 gene polymorphisms influenced the susceptibility to HBV infection in Han, Yao, Yi sub-populations in Guizhou. Result of the study suggested that IL-10-592 gene polymorphisms might serve as a risk factor to HBV infection.