Anti-microbial Activity of Urine after Ingestion of Cranberry: A Pilot Study.

We explore the anti-microbial activity of urine specimens after the ingestion of a commercial cranberry preparation. Twenty subjects without urinary infection, off antibiotics and all supplements or vitamins were recruited. The study was conducted in two phases: in phase 1, subjects collected the first morning urine prior to ingesting 900 mg of cranberry and then at 2, 4 and 6 h. In phase 2, subjects collected urine on 2 consecutive days: on Day 1 no cranberry was ingested (control specimens), on Day 2, cranberry was ingested. The pH of all urine specimens were adjusted to the same pH as that of the first morning urine specimen. Aliquots of each specimen were independently inoculated with Escherichia coli, Klebsiella pneumoniae or Candida albicans. After incubation, colony forming units/ml (CFU ml(-1)) in the control specimen was compared with CFU ml(-1) in specimens collected 2, 4 and 6 h later. Specimens showing ≥50% reduction in CFU ml(-1) were considered as having 'activity' against the strains tested. In phase 1, 7/20 (35%) subjects had anti-microbial activity against E. coli, 13/20 (65%) against K. pneumoniae and 9/20 (45%) against C. albicans in specimens collected 2-6 h after ingestion of cranberry. In phase 2, 6/9 (67%) of the subjects had activity against K. pneumoniae. This pilot study demonstrates weak anti-microbial activity in urine specimens after ingestion of a single dose of commercial cranberry. Anti-microbial activity was noted only against K. pneumoniae 2-6 h after ingestion of the cranberry preparation.

Background

Cranberry (Vaccinium macrocarpon) fruits and leaves have a long history of traditional use in folk medicine for the management of diverse conditions including urinary problems, wounds, stomach problems, diabetes, etc. Over the last decades popular interest in the use of cranberry for the prevention and treatment of urinary tract infections (UTI) has been on the rise. Sales of cranberry in 2005 exceeded $15 million and cranberry was ranked the fifth highest selling herb in the US (1).

Scientific interest in cranberry followed popular interest closely. A PubMed search over the last 36 years (1970–2006), limited to title, identified 185 papers on cranberry, of which, 22 were randomized controlled trials, and two Cochrane reviews. The majority of published controlled trials explore the effectiveness of cranberry for the prevention of UTI (2). Cochrane reviewers concluded that evidence from two well-designed studies indicates that cranberry juice decreased the number of symptomatic UTIs over a 12-month period. Few studies looked at the use of cranberry for the treatment of UTI, however well-designed studies are yet to be conducted as indicated by the 2004 Cochrane review (3). No major adverse effects or interactions were reported or identified in these reviews or more recent studies (4,5).

With mounting interest in the clinical use of cranberry, scientific curiosity about cranberry's anti-microbial mechanism of action grew. The exact anti-microbial action of cranberry remains under investigation. Initial proposed mechanism attributed the anti-microbial benefits to hippuric acid which has the potential to acidify the urine (6). Later studies confirmed that cranberry juice can lower urinary PH (7,8). More recently, studies suggested that cranberry's activity is related to the inhibition of bacterial adherence to the uroepithelium. Recent evidence suggests that fructose, found in cranberries, interferes with adhesion of type 1 fimbriated (mannose-sensitive) Escherichia coli to the uroepithelium (9), while other studies show that proanthocyanidins in cranberries inhibit the adherence of p-fimbriated (mannose-resistant) E. coli to the uroepithelium (10,11). In fact, recent studies suggest that cranberry juice irreversibly inhibits p-fimbriae preventing attachment of E. coli to the uroepithelium (12).

In addition to the anti-adhesive effect, a possible anti-microbial activity has been suggested. In effect a laboratory study verified that concentrated cranberry juice had a direct anti-microbial activity in vitro (13). However, despite the encouraging outcomes reported in the prevention studies and in laboratory studies looking at mechanism of action, evidence of direct anti-microbial activity in the urine after ingestion of cranberry has yet to be demonstrated.

To evaluate the anti-microbial effect of urine specimens after ingestion of cranberry we conducted a pilot study assaying the direct anti-microbial activity of urine specimens taken from volunteer subjects after ingestion of a commercially available cranberry product.

Methods

This study proposes to evaluate the in vitro bioactivity (anti-microbial) of urine samples after ingestion of cranberry. The protocols were approved by the institutional review board. Twenty asymptomatic adults were recruited for the entire study, all of them completed phase 1, and nine completed phase 2. The study team was blinded to the identity of the subjects. All subjects were asked to abstain from taking any cranberry, health food fruit extracts, anti-oxidants, vitamins or minerals and antibiotics for 1 and 2 weeks, respectively, prior to particpating in the studies and during specimen collection. Subjects were also asked to complete a food diary starting 3 days prior and on the day(s) of specimen collections.

Product Used

A commercially available cranberry softgel ‘TruNature’ was used in this study. Each 300 mg softgel contained of a mixture of dry whole cranberry extract (275 mg) and ShanStar Concentrate cranberry extract (25 mg). ShanStar Concentrate extract is a highly purified preparation of cranberry proanthocyanins on cross-linked polyvinylpyrrolidone (PVP) substrate. During the preparation, PVP is mixed into normal cranberry juice. The proanthocyanins and other polyphenolic compounds have a very high affinity for the PVP molecule. The PVP-cranberry (CB-PVP) is then removed from the juice by filtration and dried. HPLC analysis of ShanStar Concentrate extract shows several 100-fold increase in the concentration of the proanthocyanins and other polyphenolic compounds when compared to normal juice without the high level of sugars and organic acids usually found in cranberry juice (14). To ensure that the activity seen in the test is from cranberry and not from PVP, we conducted a small comparison of the anti-bacterial activity of PVP versus cranberry PVP (Table 1). The results confirm that the anti-bacterial activity seen is from cranberry.

Table 1.

Anti-bacterial activity of cranberry PVP versus PVP in Broth

Strains	CB PVP	Broth only	PVP broth		Cranberry PVP broth
	Batch No.	CFU ml−1	Dilution	CFU ml−1	Dilution	CFU ml−1
E. coli (ATTC 295220)	1	2 × 109	1: 2	1.7 × 108	1: 2	1 × 107
	2	3.7 × 108	1: 2	9 × 107	1: 2	5 × 106
	3	1.9 × 109	1: 2	1.8 × 108	1: 2	1 × 107
K. pneumonia (ATCC 13883)	4	4.3 × 108	1: 2	8.5 × 107	1: 2	<102
S. aureus (ATCC 29213)	4	1 × 108	1: 2	1.8 × 108	1: 2	1.6 × 101
Proteus mirablis (Clinical isolate)	4	1.9 × 108	1: 2	7.2 × 107	1: 2	2.6 × 104

Initial inoculate approximately 105 CFU ml−1. Overnight incubation at 35°C.

Study Conducted

The study was conducted in two phases. In the phase 1, urine was collected for 1 day only. On the day of the study, the first morning urine was collected as a baseline contol urine. Subjects were then asked to take a commercial cranberry product (TruNature cranberry 300 mg softgels). Each subject was asked to take three softgels (900 mg) once in the morning. Additional urine samples were then collected at 2 h inetrvals over 6 h, for a total of three samples (at 2, 4 and 6 h post-ingestion of cranberry) (Fig. 1). Each subject was asked to immediately refrigerate all samples after testing and recording each specimens’ specific gravity and PH. All specimes and data were confidentially coded. Thirteen subjects were enrolled in this phase of the study.

Figure 1.

Time line for urine collection.

In phase 2, the protocol was amended and subjects were asked to collect urine specimens for two subsequent days. On Day 1, urine was collected at 2 h intervals, as in phase 1, but without cranberry ingestion (control specimens). On Day 2, the protocol remained the same as that of phase 1, (i.e. urine was collected at 2 h intervals, as in phase 1, but with cranberry ingestion, see Fig. 1). Thus, in this phase of the study we were able to compare the anti-microbial activity in urine samples collected on 2 subsequent days with and without ingestion of cranberry. Nine subjects were tested; two subjects were already enrolled in phase 1 of the study and consented to repeat the sample collections for phase 2.

Microbiological Assays

In the laboratory, urines were filter-sterilized, aliquots of each of the specimens was then assayed for anti-microbial activity in vitro against a variety of common bacterial organisms which can be the cause of human UTI.

The pH of all urine specimens was adjusted to the same pH as the baseline control specimens and filtered. Aliquots of each specimen were independently inoculated with 102–103 cell ml−1 each of single strains of E. coli, Klebsiella pneumoniae and Candida albicans. Bacterial strains tested included E. coli ATCC 29522, K. pneumoniae ATCC 13883 and clinical strain C. albicans from University of California at Irvine medical center. Overnight cultures were incubated at 35°C on tryticase soy agar media were used as inocula.

A low inoculum of bacteria was used deliberately to mimic colonization and not an established infection. The organisms were the same strains utilized in our pilot study (13). After 24 h of incubation for E. coli and K. pneumoniae, and 48 h for C. albicans, colony forming units per milliliter (CFU ml−1) of each specimen were enumerated by subculture with quantitative plate counts in duplicate. The number of CFU ml−1 in the baseline control specimens (early morning urine specimen) were compared with CFU ml−1 in specimens collected 2, 4 and 6 h after ingestion of cranberry. Specimens that showed ≥50% reduction in CFU ml−1 compared with its controls were considered as having ‘activity’ against the strains tested.

Data Analysis

Data was analyzed in two parts: in part one data from all 20 subjects were included comparing the anti-microbial activity of samples 2, 3 and 4 (all after ingestion of cranberry) to the morning urine sample (control specimen taken before ingestion of cranberry). In part two, nine subjects who collected samples as per phase 2 protocol (2-day urine collection) were included. Morning urine specimens were used as the controls in each of the 2 days of this phase. Anti-microbial activity at 2, 4 and 6 h were compared with the morning specimen (as control) of their respective day. Presence of anti-microbial activity was defined as 50% reduction in CFU ml−1 between the first morning urine specimen (control) and subsequent specimens collected at 2, 4 and 6 h. The statistic method used was McNemar chi-squared test.

Results

Phase 1

Data from all 20 subjects were initially analyzed comparing morning urine anti-microbial activity to that seen after ingestion of cranberry. Four men and 16 women age range 36–72 years, provided urine specimens. Anti-microbial activity (≥50% reduction in CFU from control specimen) against E. coli strain was identified in 7 of the 20 (35%) subjects studied. Anti-microbial activity was also seen in 13/20 subjects (65%) for K. pneumoniae strain and in 9/20 (45%) for C. albicans. Timing of the observed activity varied and was seen at 2, 4 or 6 h: however, most activities were noted 2 h after ingestion of cranberry (Fig. 2). The activity was generally modest but ranged from <1 log10 (50%) reduction to 2 log10 (99%) reduction in CFU.

Figure 2.

Anti-microbial activity found in urine after ingestion of cranberry compared with first morning urine (phase 1). Only maximal anti-microbial activity identified for each participant is reported.

Phase 2

In the second phase of the study, nine subjects provided urine specimens, seven women and two men. Analysis of specimens provided during the second phase of the study showed no significant difference in anti-microbial activity between control (Day 1 without cranberry) and test (Day 2 with cranberry) urine specimens for E. coli and C. albicans. Anti-microbial activity against K. pneumoniae was noted in six out of nine subjects (67%) on day 2 after cranberry ingestion compared to 2/9 (22%) on day 1 (no cranberry) (Fig. 3). Due to the small sample size, the difference was not statistically significant.

Figure 3.

Anti-microbial activity found in urine with and without ingestion of cranberry on 2 subsequent days (phase 2). Only maximal anti-microbial activity identified per participant each day is reported.

No difference in activity between men and women was noted, although the number of men sampled was too small for adequate comparison. Selection of study volunteers was not stratified by age group. None of the enrolled subjects withdrew prior to completing the study. No side effects were reported throughout the study.

Discussion

Cranberry is commonly recommended for treatment/prevention of UTIs. Cochrane reviews of clinical studies concluded that evidence supports a role for cranberry in the prevention of UTIs, but not in the treatment of an established infection (3,15). Proposed mechanisms of action included anti-adherance (9–12,16,17), anti-bacterial (13), changes in urine acidity (PH) (7,8) and activation of the nitric oxide pathway. Laboratory studies identified a direct anti-bacterial activity of cranberry (13), however little is known whether this activity is maintained after ingestion. In this study, we explored the presence of anti-microbial activity in the urine of subjects after ingestion of cranberry.

For this study, we used an inoculation of 102–103 instead of the standard 105. This decision was reached after careful review of the published data and the Cochrane reviews which concluded that cranberry was more effective in the prevention (eliminate colonization) rather than treatment of UTI.

We postulated that the anti-bacterial effect is due to the anthocyanins and proanthocyanidins fractions of cranberry. Urine samples were collected at 2, 4 and 6 h after ingestion of cranberry. A recent study by Ohnishi et al. (18) demonstrated the urinary levels of anthocyanins reached a maximum between 3 and 6 h after ingestion of cranberry.

Our study suggests that anti-bacterial activity against E. coli after ingestion of cranberry was not significant. This confirms findings from two recent published studies exploring the anti-bacterial activity of urine after ingestion of cranberry (14,19). Both studies adjusted the specimens for urine PH. In the study by Monroy-Torres et al. (14), 20 young (19–24 years old) healthy women provided urine samples before and 3 h after taking 250 ml of cranberry juice. Urine samples were incubated for 1–2 h only then plated. No significant drop in the number of E. coli colonies were seen. In the study by Tong et al., 10 young healthy Chinese adults (mean age 20.4 ± 1.2 years) collected urine at 3 h intervals on 2 consecutive days. On day 2 subjects drank 750 ml of cranberry juice. Urine samples were inoculated with 2.8 × 107 E. coli and incubated for 24 h. No statistically significant differences were observed between the control (day 1) and urine specimens collected on day 2 after ingestion of the cranberry juice (19). Even though the studies used different forms (juice and tablets) and doses of cranberry, the final result was the same regardless of the time of urine collection after cranberry ingestion.

In this study we also explored the presence of anti-microbial activity against less common causes of UTI, such as C. albicans and K. pneumonia. No significant drop in the number of colonies was observed for Candida after ingestion of cranberry. However, anti-microbial activity against K. pneumonia was detected in the urine of six out of nine subjects after ingestion of the cranberry capsules. It is of interest that the occurrence of anti-microbial activity against K. pneumonia was noted when samples were compared in phase 1 of the study (using first morning urine specimen as control), and phase 2 (comparing samples from both days to their respective morning control specimen). Timing of the observed activity in all three strains tested varied among the 2, 4 or 6 h specimens; however, activity was mainly observed in the urine specimens collected 2 h after ingestion of the capsules. Based on this result one can postulate that the anti-microbial activity found in this study against K. pneumoniae is probably due to factors other than the anti-adherence activity reported for E. coli.

During phase 1, 35%, 45% and 65% of specimens collected form the 20 study subjects showed anti-microbial activity in the urine against E. coli, C. albicans and K. pneumoniae respectively. This activity occurred 2–6 h after ingestion of cranberry capsules. In the second phase, 23%, 33% and 67% of the specimens showed some anti-microbial activity against E. coli, C. albicans and K. pneumoniae respectively. It is of interest that the most frequent occurrence of anti-microbial activity occurred in both phases against K. pneumoniae. Thus, in the CB-PVP there might be a different anti-microbial factor inhibiting K. pneumoniae other than the anti-adherence factor reported in previous studiers with E. coli.

We attempted to maximize the sensitivity of our essay by using a low colony count and defining activity as a 50% reduction in colony counts. We did this as we believe the anti-microbial activity of natural products may be more subtle than those noted in commercial antibiotics where a two log reduction in colony count may be expected.

Further studies are warranted to better understand the nature of the factors responsible for the anti-microbial activity in urines following cranberry capsules ingestion, as well as to better understand the bacterial spectrum of these factors and how they might be enhanced including a determination of optimal dosing. A study with higher concentration of CB-PVP and testing of bacterial adhesion is in progress.

Conclusion

Following ingestion of cranberry, anti-microbial activity was seen with K. pneumoniae. Additional studies are also needed to further explore the anti-microbial activity using different formulations (juice, capsule) and doses. Other studies should also explore the possibility of optimizing the anti-microbial activity using cranberry in combination with other fruits or supplements.

Competing Interests: Dr Edward Shanbrom is the inventor of Shanstar and holds the patent for it.