| Literature DB >> 18952061 |
Qing Liu1, Xiaoqing He, Yongsheng Liu, Bingbing Du, Xiaoyan Wang, Weisheng Zhang, Pengfei Jia, Jingmei Dong, Jianxiu Ma, Xiaohu Wang, Sha Li, Hong Zhang.
Abstract
Oxidative damage is an important mechanism in X-ray-induced cell death. Radiolysis of water molecules is a source of reactive oxygen species (ROS) that contribute to X-ray-induced cell death. In this study, we showed by ROS detection and a cell survival assay that NADPH oxidase has a very important role in X-ray-induced cell death. Under X-ray irradiation, the upregulation of the expression of NADPH oxidase membrane subunit gp91(phox) was dose-dependent. Meanwhile, the cytoplasmic subunit p47(phox) was translocated to the cell membrane and localized with p22(phox) and gp91(phox) to form reactive NADPH oxidase. Our data suggest, for the first time, that NADPH oxidase-mediated generation of ROS is an important contributor to X-ray-induced cell death. This suggests a new target for combined gene transfer and radiotherapy.Entities:
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Year: 2008 PMID： 18952061 DOI： 10.1016/j.bbrc.2008.10.067
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575