| Literature DB >> 18951880 |
Jerzy Orlowski1, Mehari Tesfazgi Mebrhatu, Chris W Michiels, Janusz M Bujnicki, Abram Aertsen.
Abstract
The Mrr protein of Escherichia coli K12 is a cryptic Type IV restriction endonuclease whose activity appears to be triggered by high pressure stress. In this report we used high pressure to isolate and analyze several Mrr mutants, and generated a new structural model of the Mrr protein. The activity of a number of spontaneous and strategically constructed Mrr mutants is discussed in the light of this model, providing a first insight into the structure-function relationships of the Mrr enzyme.Entities:
Mesh：
Substances：
Year: 2008 PMID： 18951880 DOI： 10.1016/j.bbrc.2008.10.064
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575