| Literature DB >> 18951190 |
Jean-Christophe Amé1, Antoinette Hakmé, Delphine Quenet, Elise Fouquerel, Françoise Dantzer, Valérie Schreiber.
Abstract
Poly(ADP-ribosyl)ation is a posttranslational modification of proteins in higher eukaryotes mediated by poly(ADP-ribose) polymerases (PARPs) that is involved in many physiological processes such as DNA repair, transcription, cell division, and cell death. Biochemical studies together with PARP-1- or PARP-2-deficient cellular and animal models have revealed the redundant but also complementary functions of the two enzymes in the surveillance and maintenance of genome integrity. Poly(ADP-ribose) is degraded by the endo- and exo-glycosidase activities of poly(ADP-ribose) glycohydrolase (PARG). In this chapter, biochemical and immunofluorescence methods are described for detecting and assaying PARPs and PARG.Entities:
Mesh：
Substances：
Year: 2009 PMID： 18951190 DOI： 10.1007/978-1-60327-461-6_15
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745