| Literature DB >> 18951180 |
Catherine Schurra1, Aaron Bensimon.
Abstract
Molecular combing is a process whereby single DNA molecules bind by their extremities to a silanised surface and are then uniformly stretched and aligned by a receding air/water interface (1). This method, with a high resolution ranging from a few kilobases to megabases, has many applications in the field of molecular cytogenetics, allowing structural and functional analysis at the genome level. Here we describe protocols for preparing DNA for combing and for the use of fluorescent hybridisation (FH) applied to combed DNA to conduct physical mapping or genomic structural analysis. We also present the methodology for visualising and studying DNA replication using combed DNA.Entities:
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Year: 2009 PMID： 18951180 DOI： 10.1007/978-1-60327-461-6_5
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745