Literature DB >> 18948202

Recombinant bacterial RNA polymerase: preparation and applications.

Konstantin Kuznedelov1, Konstantin Severinov.   

Abstract

Availability of DNA-dependent RNA polymerase from various bacteria is a key for setting up specific in vitro transcription systems necessary for understanding species-specific transcription regulation. We describe here two main strategies for recombinant RNA polymerase preparation-through in vitro reconstitution and heterologous co-overproduction in Escherichia coli. Both strategies can be used for preparation of large amounts of RNA polymerases from any bacteria for which sequences of rpo (RNA polymerase) genes are known.

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Year:  2008        PMID： 18948202     DOI： 10.1016/j.ymeth.2008.10.007

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  4 in total

1.  Characterization and in vivo regulon determination of an ECF sigma factor and its cognate anti-sigma factor in Nostoc punctiforme.

Authors:  Nicole Bell; Jamie J Lee; Michael L Summers
Journal:  Mol Microbiol       Date:  2017-02-15       Impact factor: 3.501

2.  Overproduction and purification of highly active recombinant Pseudomonas aeruginosa str. PAO1 RNA polymerase holoenzyme complex.

Authors:  Derrick Afful; Liming Cai; Cory Momany
Journal:  Protein Expr Purif       Date:  2019-07-04       Impact factor: 1.650

3.  In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme.

Authors:  Haruichi Asahara; Shaorong Chong
Journal:  Nucleic Acids Res       Date:  2010-05-10       Impact factor: 16.971

4.  A PorX/PorY and σP Feedforward Regulatory Loop Controls Gene Expression Essential for Porphyromonas gingivalis Virulence.

Authors:  Chizhou Jiang; Dezhi Yang; Tangsiyuan Hua; Zichun Hua; Wei Kong; Yixin Shi
Journal:  mSphere       Date:  2021-05-28       Impact factor: 4.389
  4 in total

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