Heidi Snethen1, Seth Love, Neil Scolding. 1. Department of Neurology, University of Bristol Institute of Clinical Neurosciences, Frenchay Hospital, Bristol BS161LE, UK.
Abstract
AIMS: Spontaneous tissue repair occurs in multiple sclerosis (MS), but the origin of remyelinating cells remains obscure. Here we explore the hypothesis that endogenous neural precursors are involved in MS disease processes. MATERIALS & METHODS: We studied postmortem brain and spinal cord samples from MS patients using immunocytochemical techniques. RESULTS: We show that cells co-positive for nestin and musashi-1 are not merely present in lesions, but found in markedly increased numbers (up to fivefold). Small numbers of nestin-positive cells show direct evidence of proliferation, co-staining for Ki67; some also coexpress glial fibrillary acidic protein or oligodendrocyte progenitor markers (NG-2 or PDGF-alpha receptor), or the early neuronal marker doublecortin, consistent with transition from neural precursors. CONCLUSIONS: These findings suggest that endogenous neural precursors react to disease processes in MS.
AIMS: Spontaneous tissue repair occurs in multiple sclerosis (MS), but the origin of remyelinating cells remains obscure. Here we explore the hypothesis that endogenous neural precursors are involved in MS disease processes. MATERIALS & METHODS: We studied postmortem brain and spinal cord samples from MS patients using immunocytochemical techniques. RESULTS: We show that cells co-positive for nestin and musashi-1 are not merely present in lesions, but found in markedly increased numbers (up to fivefold). Small numbers of nestin-positive cells show direct evidence of proliferation, co-staining for Ki67; some also coexpress glial fibrillary acidic protein or oligodendrocyte progenitor markers (NG-2 or PDGF-alpha receptor), or the early neuronal marker doublecortin, consistent with transition from neural precursors. CONCLUSIONS: These findings suggest that endogenous neural precursors react to disease processes in MS.
Authors: Alexandra M Nicaise; Laura J Wagstaff; Cory M Willis; Carolyn Paisie; Harshpreet Chandok; Paul Robson; Valentina Fossati; Anna Williams; Stephen J Crocker Journal: Proc Natl Acad Sci U S A Date: 2019-03-25 Impact factor: 11.205
Authors: Ellen Iacobaeus; Rachael V Sugars; Anton Törnqvist Andrén; Jessica J Alm; Hong Qian; Janek Frantzen; Jia Newcombe; Kanar Alkass; Henrik Druid; Matteo Bottai; Matias Röyttä; Katarina Le Blanc Journal: Stem Cells Transl Med Date: 2017-09-23 Impact factor: 6.940