Literature DB >> 18945844

Colony multiplex-tandem PCR for rapid, accurate identification of fungal cultures.

Anna Lau1, Tania C Sorrell, OkCha Lee, Keith Stanley, Catriona Halliday.   

Abstract

We developed a multiplex tandem PCR (MT-PCR) assay for the rapid identification of 16 fungi directly from culture. MT-PCR results were concordant with phenotypic identification for all cultures studied (n = 183). The colony MT-PCR assay was rapid (<2 h), sensitive, and specific in identifying fungal pathogens directly from primary isolation plates.

Mesh：

Year:  2008        PMID： 18945844      PMCID： PMC2593288          DOI： 10.1128/JCM.01411-08

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  19 in total

1.  Polymorphic internal transcribed spacer region 1 DNA sequences identify medically important yeasts.

Authors:  Y C Chen; J D Eisner; M M Kattar; S L Rassoulian-Barrett; K Lafe; U Bui; A P Limaye; B T Cookson
Journal:  J Clin Microbiol       Date:  2001-11       Impact factor: 5.948

2.  Rapid identification of fungi by sequencing the ITS1 and ITS2 regions using an automated capillary electrophoresis system.

Authors:  T M Pryce; S Palladino; I D Kay; G W Coombs
Journal:  Med Mycol       Date:  2003-10       Impact factor: 4.076

3.  Species identification of medically important fungi by use of real-time LightCycler PCR.

Authors:  Min-Chih Hsu; Kuo-Wei Chen; Hsiu-Jung Lo; Yee-Chun Chen; Mei-Hui Liao; Yu-Hui Lin; Shu-Ying Li
Journal:  J Med Microbiol       Date:  2003-12       Impact factor: 2.472

4.  Internal transcribed spacer sequencing versus biochemical profiling for identification of medically important yeasts.

Authors:  D E Ciardo; G Schär; E C Böttger; M Altwegg; P P Bosshard
Journal:  J Clin Microbiol       Date:  2006-01       Impact factor: 5.948

5.  Phenotypic and molecular characterization of Candida nivariensis sp. nov., a possible new opportunistic fungus.

Authors:  Julia Alcoba-Flórez; Sebastián Méndez-Alvarez; Josep Cano; Josep Guarro; Eduardo Pérez-Roth; María del Pilar Arévalo
Journal:  J Clin Microbiol       Date:  2005-08       Impact factor: 5.948

6.  Development of a DNA microarray for detection and identification of fungal pathogens involved in invasive mycoses.

Authors:  Dirk M Leinberger; Ulrike Schumacher; Ingo B Autenrieth; Till T Bachmann
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

7.  Taxonomic position of clinical isolates of Candida famata.

Authors:  A Nishikawa; H Tomomatsu; T Sugita; R Ikeda; T Shinoda
Journal:  J Med Vet Mycol       Date:  1996 Nov-Dec

8.  Differentiation between Debaryomyces hansenii/Candida famata complex and Candida guilliermondii by polymerase chain reaction.

Authors:  A Nishikawa; T Sugita; T Shinoda
Journal:  FEMS Immunol Med Microbiol       Date:  1997-10

9.  Rapid identification of pathogenic fungi directly from cultures by using multiplex PCR.

Authors:  Guizhen Luo; Thomas G Mitchell
Journal:  J Clin Microbiol       Date:  2002-08       Impact factor: 5.948

10.  Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection.

Authors:  Keith K Stanley; Elektra Szewczuk
Journal:  Nucleic Acids Res       Date:  2005-11-24       Impact factor: 16.971
View more
  8 in total

1.  Fungal endophytic communities in grapevines (Vitis vinifera L.) respond to crop management.

Authors:  Michael Pancher; Marco Ceol; Paola Elisa Corneo; Claudia Maria Oliveira Longa; Sohail Yousaf; Ilaria Pertot; Andrea Campisano
Journal:  Appl Environ Microbiol       Date:  2012-04-06       Impact factor: 4.792

2.  Comparison of whole blood, serum, and plasma for early detection of candidemia by multiplex-tandem PCR.

Authors:  Anna Lau; Catriona Halliday; Sharon C-A Chen; E Geoffrey Playford; Keith Stanley; Tania C Sorrell
Journal:  J Clin Microbiol       Date:  2009-12-30       Impact factor: 5.948

3.  Candida colonization as a risk marker for invasive candidiasis in mixed medical-surgical intensive care units: development and evaluation of a simple, standard protocol.

Authors:  Anna F Lau; Masrura Kabir; Sharon C-A Chen; E Geoffrey Playford; Deborah J Marriott; Michael Jones; Jeffrey Lipman; Emma McBryde; Thomas Gottlieb; Winston Cheung; Ian Seppelt; Jonathan Iredell; Tania C Sorrell
Journal:  J Clin Microbiol       Date:  2015-02-11       Impact factor: 5.948

4.  Development and validation of a multiplex PCR for detection of Scedosporium spp. in respiratory tract specimens from patients with cystic fibrosis.

Authors:  Azian Harun; Christopher C Blyth; Felix Gilgado; Peter Middleton; Sharon C-A Chen; Wieland Meyer
Journal:  J Clin Microbiol       Date:  2011-02-16       Impact factor: 5.948

5.  Development of a Real-Time PCR Assay to Identify and Distinguish between Cryptococcus neoformans and Cryptococcus gattii Species Complexes.

Authors:  Enoch Tay; Sharon C-A Chen; Wendy Green; Ronald Lopez; Catriona L Halliday
Journal:  J Fungi (Basel)       Date:  2022-04-29

6.  Molecular techniques for pathogen identification and fungus detection in the environment.

Authors:  Clement K M Tsui; James Woodhall; Wen Chen; C André Lévesque; Anna Lau; Cor D Schoen; Christiane Baschien; Mohammad J Najafzadeh; G Sybren de Hoog
Journal:  IMA Fungus       Date:  2011-11-18       Impact factor: 3.515

7.  PCR on yeast colonies: an improved method for glyco-engineered Saccharomyces cerevisiae.

Authors:  Christine Bonnet; Céline Rigaud; Emilie Chanteclaire; Claire Blandais; Emilie Tassy-Freches; Christelle Arico; Christophe Javaud
Journal:  BMC Res Notes       Date:  2013-05-20

Review 8.  Diagnosis of Fusarium Infections: Approaches to Identification by the Clinical Mycology Laboratory.

Authors:  Anne D van Diepeningen; Balázs Brankovics; Jearidienne Iltes; Theo A J van der Lee; Cees Waalwijk
Journal:  Curr Fungal Infect Rep       Date:  2015
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.