Literature DB >> 18938124

Protocols for nuclei isolation and nuclear protein extraction from the resurrection plant Xerophyta viscosa for proteomic studies.

Kamal Omer Abdalla1, Jennifer Ann Thomson, Muhammad Suhail Rafudeen.   

Abstract

The plant nucleus is an important subcellular organelle but the isolation of pure and enriched nuclei from plants and subsequent extraction of nuclear proteins for proteomic studies is challenging. Here, we present protocols for nuclei isolation and nuclear protein extraction from the resurrection plant, Xerophyta viscosa, and show optimization and modification of the most critical steps.

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Year:  2008        PMID： 18938124     DOI： 10.1016/j.ab.2008.09.049

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  9 in total

1.  Interactome analysis of transcriptional coactivator multiprotein bridging factor 1 unveils a yeast AP-1-like transcription factor involved in oxidation tolerance of mycopathogen Beauveria bassiana.

Authors:  Xin-Ling Chu; Wei-Xia Dong; Jin-Li Ding; Ming-Guang Feng; Sheng-Hua Ying
Journal:  Curr Genet       Date:  2017-09-04       Impact factor: 3.886

2.  Arabidopsis PHYTOCHROME INTERACTING FACTOR proteins promote phytochrome B polyubiquitination by COP1 E3 ligase in the nucleus.

Authors:  In-Cheol Jang; Rossana Henriques; Hak Soo Seo; Akira Nagatani; Nam-Hai Chua
Journal:  Plant Cell       Date:  2010-07-06       Impact factor: 11.277

3.  Tethered Chromosome Conformation Capture Sequencing in Triticeae: A Valuable Tool for Genome Assembly.

Authors:  Axel Himmelbach; Ines Walde; Martin Mascher; Nils Stein
Journal:  Bio Protoc       Date:  2018-08-05

4.  Cadmium toxicity and its relationship with disturbances in the cytoskeleton, cell cycle and chromosome stability.

Authors:  Daniel Pizzaia; Marina Lima Nogueira; Mateus Mondin; Marcia Eugenia Amaral Carvalho; Fernando Angelo Piotto; Millor Fernandes Rosario; Ricardo Antunes Azevedo
Journal:  Ecotoxicology       Date:  2019-09-09       Impact factor: 2.823

5.  Recognition of CCA1 alternative protein isoforms during temperature acclimation.

Authors:  Shijia Zhang; Huili Liu; Li Yuan; Xiaojing Li; Lingbao Wang; Xiaodong Xu; Qiguang Xie
Journal:  Plant Cell Rep       Date:  2021-01-04       Impact factor: 4.570

6.  Isolation of nuclear proteins from flax (Linum usitatissimum L.) seed coats for gene expression regulation studies.

Authors:  Sullivan Renouard; Corbin Cyrielle; Tatiana Lopez; Frédéric Lamblin; Eric Lainé; Christophe Hano
Journal:  BMC Res Notes       Date:  2012-01-09

7.  Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families.

Authors:  Sidona Sikorskaite; Minna-Liisa Rajamäki; Danas Baniulis; Vidmantas Stanys; Jari Pt Valkonen
Journal:  Plant Methods       Date:  2013-07-26       Impact factor: 4.993

8.  Aberrant splicing in maize rough endosperm3 reveals a conserved role for U12 splicing in eukaryotic multicellular development.

Authors:  Christine M Gault; Federico Martin; Wenbin Mei; Fang Bai; Joseph B Black; W Brad Barbazuk; A Mark Settles
Journal:  Proc Natl Acad Sci U S A       Date:  2017-02-27       Impact factor: 11.205

9.  Nuclear proteome response to cell wall removal in rice (Oryza sativa).

Authors:  Hana Mujahid; Feng Tan; Jian Zhang; Babi Ramesh Reddy Nallamilli; Ken Pendarvis; Zhaohua Peng
Journal:  Proteome Sci       Date:  2013-06-19       Impact factor: 2.480
  9 in total

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