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Literature DB >> 18931785

Single molecule studies of homologous recombination.

Abstract

Single molecule methods offer an unprecedented opportunity to examine complex macromolecular reactions that are obfuscated by ensemble averaging. The application of single molecule techniques to study DNA processing enzymes has revealed new mechanistic details that are unobtainable from bulk biochemical studies. Homologous DNA recombination is a multi-step pathway that is facilitated by numerous enzymes that must precisely and rapidly manipulate diverse DNA substrates to repair potentially lethal breaks in the DNA duplex. In this review, we present an overview of single molecule assays that have been developed to study key aspects of homologous recombination and discuss the unique information gleaned from these experiments.

Entities: Chemical Disease Gene Mutation Species

Mesh：

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Year: 2008 PMID： 18931785 PMCID： PMC2726709 DOI： 10.1039/b811681b

Source DB: PubMed Journal: Mol Biosyst ISSN： 1742-2051

129 in total

Review 1. Single-molecule fluorescence spectroscopy and microscopy of biomolecular motors.

Authors: Erwin J G Peterman; Hernando Sosa; W E Moerner
Journal: Annu Rev Phys Chem Date: 2004 Impact factor: 12.703

2. Choreography of the DNA damage response: spatiotemporal relationships among checkpoint and repair proteins.

Authors: Michael Lisby; Jacqueline H Barlow; Rebecca C Burgess; Rodney Rothstein
Journal: Cell Date: 2004-09-17 Impact factor: 41.582

3. Formation of heteroduplex DNA promoted by the combined activities of Escherichia coli recA and recBCD proteins.

Authors: L J Roman; S C Kowalczykowski
Journal: J Biol Chem Date: 1989-11-05 Impact factor: 5.157

Review 4. Structure and mechanism of Escherichia coli RecA ATPase.

Authors: Charles E Bell
Journal: Mol Microbiol Date: 2005-10 Impact factor: 3.501

5. Validating the significance of genomic properties of Chi sites from the distribution of all octamers in Escherichia coli.

Authors: Kazuharu Arakawa; Reina Uno; Yoichi Nakayama; Masaru Tomita
Journal: Gene Date: 2007-01-12 Impact factor: 3.688

6. Direct observation of individual RecA filaments assembling on single DNA molecules.

Authors: Roberto Galletto; Ichiro Amitani; Ronald J Baskin; Stephen C Kowalczykowski
Journal: Nature Date: 2006-09-20 Impact factor: 49.962

7. Origins and evolution of the recA/RAD51 gene family: evidence for ancient gene duplication and endosymbiotic gene transfer.

Authors: Zhenguo Lin; Hongzhi Kong; Masatoshi Nei; Hong Ma
Journal: Proc Natl Acad Sci U S A Date: 2006-06-23 Impact factor: 11.205

8. Fluorescent human RAD51 reveals multiple nucleation sites and filament segments tightly associated along a single DNA molecule.

Authors: Mauro Modesti; Dejan Ristic; Thijn van der Heijden; Cees Dekker; Joost van Mameren; Erwin J G Peterman; Gijs J L Wuite; Roland Kanaar; Claire Wyman
Journal: Structure Date: 2007-05 Impact factor: 5.006

9. Rad54 protein stimulates heteroduplex DNA formation in the synaptic phase of DNA strand exchange via specific interactions with the presynaptic Rad51 nucleoprotein filament.

Authors: J A Solinger; G Lutz; T Sugiyama; S C Kowalczykowski; W D Heyer
Journal: J Mol Biol Date: 2001-04-13 Impact factor: 5.469

Single molecule studies of homologous recombination.

Review 1. Single-molecule fluorescence spectroscopy and microscopy of biomolecular motors.

2. Choreography of the DNA damage response: spatiotemporal relationships among checkpoint and repair proteins.

3. Formation of heteroduplex DNA promoted by the combined activities of Escherichia coli recA and recBCD proteins.

Review 4. Structure and mechanism of Escherichia coli RecA ATPase.

5. Validating the significance of genomic properties of Chi sites from the distribution of all octamers in Escherichia coli.

6. Direct observation of individual RecA filaments assembling on single DNA molecules.

7. Origins and evolution of the recA/RAD51 gene family: evidence for ancient gene duplication and endosymbiotic gene transfer.

8. Fluorescent human RAD51 reveals multiple nucleation sites and filament segments tightly associated along a single DNA molecule.

9. Rad54 protein stimulates heteroduplex DNA formation in the synaptic phase of DNA strand exchange via specific interactions with the presynaptic Rad51 nucleoprotein filament.

10. Rad54, a Swi2/Snf2-like recombinational repair protein, disassembles Rad51:dsDNA filaments.

Review 1. How RecBCD enzyme and Chi promote DNA break repair and recombination: a molecular biologist's view.

Review 2. High-throughput single-molecule studies of protein-DNA interactions.

Review 3. Studying genomic processes at the single-molecule level: introducing the tools and applications.

Review 4. Eukaryotic resectosomes: A single-molecule perspective.

5. Supported lipid bilayers and DNA curtains for high-throughput single-molecule studies.

6. Direct observation of twisting steps during Rad51 polymerization on DNA.