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Literature DB >> 189317

Enzymatic synthesis of oligonucleotides of defined sequence: synthesis of a segment of yeast iso-1-cytochrome c gene.

Abstract

The deoxyribooligonucleotide, d(pT-T-A-G-C-A-G-A-A-C-C-G-G), constituting a segment of yeast iso-1-cytochrome c gene, has been synthesized by a combination of chemical and primarily enzymatic methods. The starting primer, d(pT-T-A-G1, was chemically synthesized by the phosphodiester method and was extended stepwise, by reactions catalyzed by polynucleotide phosphorylase.

Entities: Chemical Gene Mutation Species

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Year: 1977 PMID： 189317 PMCID： PMC393204 DOI： 10.1073/pnas.74.1.96

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

11 in total

1. THE EFFECT OF UREA, FORMAMIDE, AND GLYCOLS ON THE SECONDARY BINDING FORCES IN THE ION-EXCHANGE CHROMATOGRAPHY OF POLYNUCLEOTIDES OF DEAE-CELLULOSE.

Authors: R V TOMLINSON; G M TENER
Journal: Biochemistry Date: 1963 Jul-Aug Impact factor: 3.162

2. Enzymatic synthesis of oligonucleotides of defined sequence. Addition of short blocks of nucleotide residues to oligonucleotide primers.

Authors: S Gillam; K Waterman; M Smith
Journal: Nucleic Acids Res Date: 1975-05 Impact factor: 16.971

3. Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence.

Authors: S Gilham; M Smith
Journal: Nat New Biol Date: 1972-08-23

4. Studies on polynucleotides. 103. Total synthesis of the structural gene for an alanine transfer ribonucleic acid from yeast.

Authors: H G Khorana; K L Agarwal; H Büchi; M H Caruthers; N K Gupta; K Kleppe; A Kumar; E Otsuka; U L RajBhandary; J H Van de Sande; V Sgaramella; T Terao; H Weber; T Yamada
Journal: J Mol Biol Date: 1972-12-28 Impact factor: 5.469

5. Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Properties of the enzyme.

Authors: S Gillam; M Smith
Journal: Nucleic Acids Res Date: 1974-12 Impact factor: 16.971

6. Anion-exchange chromatography on reversed-phase columns: isolation and assay of nucleosides, nucleotides, and oligonucleotides from nucleic acids and from cytoplasm.

Authors: R P Singhal
Journal: Biochim Biophys Acta Date: 1973-08-10

7. Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose.

Authors: G G Brownlee; F Sanger
Journal: Eur J Biochem Date: 1969-12

8. Separation of oligonucleotides by reversed-phase chromatography.

Authors: B Z Egan
Journal: Biochim Biophys Acta Date: 1973-03-19

9. Catalysis of DNA joining by bacteriophage T4 RNA ligase.

Authors: T J Snopek; A Sugino; K L Agarwal; N R Cozzarelli
Journal: Biochem Biophys Res Commun Date: 1976-01-26 Impact factor: 3.575

10. Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Deoxyribo-oligonucleotide synthesis.

Authors: S Gillam; K Waterman; M Doel; M Smith
Journal: Nucleic Acids Res Date: 1974-12 Impact factor: 16.971

5 in total

1. Enzymatic synthesis of oligodeoxyribonucleotides of defined sequence. Polynucleotide phosphorylase catalysed synthesis using pyrimidine analog-containing deoxyribonucleoside 5'-diphosphates.

Authors: E M Trip; M Smith
Journal: Nucleic Acids Res Date: 1978-05 Impact factor: 16.971

2. Enzymatic synthesis of oligodeoxyribonucleotides of defined sequence. Polynucleotide phosphorylase catalysed addition of deoxyribonucleotides to primers which are good or poor acceptors.