| Literature DB >> 18931299 |
Julia P Galkiewicz1, Christina A Kellogg.
Abstract
PCR amplification of pure bacterial DNA is vital to the study of bacterial interactions with corals. Commonly used Bacteria-specific primers 8F and 27F paired with the universal primer 1492R amplify both eukaryotic and prokaryotic rRNA genes. An alternative primer set, 63F/1542R, is suggested to resolve this problem.Mesh:
Substances:
Year: 2008 PMID: 18931299 PMCID: PMC2607152 DOI: 10.1128/AEM.01303-08
Source DB: PubMed Journal: Appl Environ Microbiol ISSN: 0099-2240 Impact factor: 4.792