| Literature DB >> 18931078 |
Fabiana Magalhães Coelho1, Maria Rosa Quaresma Bomfim2, Fabíola de Andrade Caxito1, Natália Almeida Ribeiro1, Marcela Miranda Luppi1, Érica Azevedo Costa1, Maria Emilia Oliveira1, Flávio Guimarães Da Fonseca1, Mauricio Resende1.
Abstract
A nested-PCR (n-PCR) was used to detect feline leukemia virus (FeLV) proviral DNA in blood samples from 464 sick and 608 healthy domestic cats (Felis catus) selected by convenience, and a significantly high prevalence of FeLV infection was observed. n-PCR results revealed the presence of FeLV proviral DNA in 47.2 % of sick cats and 47.4 % of healthy cats. Phylogenetic analysis revealed that FeLV samples from healthy or sick cats were grouped into separate clades. We determined FeLV subgroups by an n-PCR based on the envelope (env) gene. The partial env gene of FeLV Minas Gerais (MG) samples were compared to various exogenous FeLV isolates and endogenous (enFeLV) provirus from the same region. FeLV-B MG samples were more similar to endogenous sequences and to natural FeLV-B isolates than to either FeLV-A or FeLV-C. The results revealed the circulation of FeLV-B in large populations of urban domestic cats in Brazil.Entities:
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Year: 2008 PMID： 18931078 DOI： 10.1099/vir.0.2008/003855-0
Source DB: PubMed Journal: J Gen Virol ISSN： 0022-1317 Impact factor: 3.891