OBJECTIVE: To investigate the pathogenesis of endometriotic pain. DESIGN: Retrospective nonrandomized immunohistochemical study. SETTING: A university hospital, Department of Gynecology. PATIENT(S): Twenty human endometriotic specimens were selected from different lesions including ovarian endometrioma, peritoneal lesion, and deep infiltrating lesion. Premenopausal women with histologically diagnosed endometriosis were selected (mean age 39 years; range, 25-53 years). The chief complaint was dysmenorrhea, dyschezia, and dyspareunia. A rat endometriosis model was induced in 10 SLC-Sprague-Dawley rats (8 weeks old) by surgical autotransplantation of the uterus. INTERVENTION(S): Immunohistochemical staining of endometriotic specimens for alpha-smooth muscle actin (ASMA), neural cell adhesion molecule (NCAM), and nerve growth factor (NGF) expression. MAIN OUTCOME MEASURE(S): Comparison of the immunoreactive staining of ASMA, NCAM, and NGF expression in human endometriosis and a rat endometriosis model. RESULT(S): Morphological analysis revealed thick interstitium in both human and rat endometriotic lesions. The major components of fibrotic interstitium are smooth muscle cells, stained by anti-ASMA antibody, and nerve cells, stained by anti-NCAM antibody. Inflammatory cells are also present (e.g., macrophages and lymphocytes) as revealed by anti-NGF antibody staining. CONCLUSION(S): These results suggest that the contraction of smooth muscle cells and the hyperalgia derived from innervation in the interstitial area is related to pain in endometriosis.
OBJECTIVE: To investigate the pathogenesis of endometriotic pain. DESIGN: Retrospective nonrandomized immunohistochemical study. SETTING: A university hospital, Department of Gynecology. PATIENT(S): Twenty human endometriotic specimens were selected from different lesions including ovarian endometrioma, peritoneal lesion, and deep infiltrating lesion. Premenopausal women with histologically diagnosed endometriosis were selected (mean age 39 years; range, 25-53 years). The chief complaint was dysmenorrhea, dyschezia, and dyspareunia. A ratendometriosis model was induced in 10 SLC-Sprague-Dawley rats (8 weeks old) by surgical autotransplantation of the uterus. INTERVENTION(S): Immunohistochemical staining of endometriotic specimens for alpha-smooth muscle actin (ASMA), neural cell adhesion molecule (NCAM), and nerve growth factor (NGF) expression. MAIN OUTCOME MEASURE(S): Comparison of the immunoreactive staining of ASMA, NCAM, and NGF expression in humanendometriosis and a ratendometriosis model. RESULT(S): Morphological analysis revealed thick interstitium in both human and ratendometriotic lesions. The major components of fibrotic interstitium are smooth muscle cells, stained by anti-ASMA antibody, and nerve cells, stained by anti-NCAM antibody. Inflammatory cells are also present (e.g., macrophages and lymphocytes) as revealed by anti-NGF antibody staining. CONCLUSION(S): These results suggest that the contraction of smooth muscle cells and the hyperalgia derived from innervation in the interstitial area is related to pain in endometriosis.
Authors: Yana B Aznaurova; Marat B Zhumataev; Tiffany K Roberts; Alexander M Aliper; Alex A Zhavoronkov Journal: Reprod Biol Endocrinol Date: 2014-06-13 Impact factor: 5.211
Authors: A Nishimoto-Kakiuchi; S Netsu; S Matsuo; S Hayashi; T Ito; S Okabayashi; L Yasmin; K Yuzawa; O Kondoh; A Kato; M Suzuki; R Konno; T Sankai Journal: Hum Reprod Date: 2016-09-02 Impact factor: 6.918