OBJECTIVE: To evaluate the reliability and applicability of the spot-counting system (Cytovision Spot AX workstation) which offers an alternative to the tedious manual analysis of sperm fluorescence in situ hybridization (FISH). DESIGN: Manual and automatic analyses were performed and compared. SETTING: Universitat Autònoma de Barcelona. PATIENT(S): Twenty-four men who asked for information on infertility showing different seminal parameters. INTERVENTION(S): A semen sample for each patient was collected and prepared for FISH. MAIN OUTCOME MEASURE(S): A dual-color FISH using specific probes for chromosomes 13 and 21 and a triple-color FISH with centromeric probes for chromosomes 18, X, and Y were used (Vysis). Standard FISH analysis was carried out. Automatic analysis was subsequently performed using a Spot AX system. RESULT(S): Overall, we performed 120 comparisons. In 116 out of 120 (96.67%), the percentage of anomalies reported using manual counting fell within the incidence detected using the automatic system. In the remaining comparisons, statistical differences were detected (4 out of 120; 3.33%). Time consumed by the automatic analysis was always higher than the manual one, being influenced by the characteristics of the preparations. CONCLUSION(S): The spot-counting system has potential, but before the service is ready to be offered, we still need to overcome some limitations associated with it.
OBJECTIVE: To evaluate the reliability and applicability of the spot-counting system (Cytovision Spot AX workstation) which offers an alternative to the tedious manual analysis of sperm fluorescence in situ hybridization (FISH). DESIGN: Manual and automatic analyses were performed and compared. SETTING: Universitat Autònoma de Barcelona. PATIENT(S): Twenty-four men who asked for information on infertility showing different seminal parameters. INTERVENTION(S): A semen sample for each patient was collected and prepared for FISH. MAIN OUTCOME MEASURE(S): A dual-color FISH using specific probes for chromosomes 13 and 21 and a triple-color FISH with centromeric probes for chromosomes 18, X, and Y were used (Vysis). Standard FISH analysis was carried out. Automatic analysis was subsequently performed using a Spot AX system. RESULT(S): Overall, we performed 120 comparisons. In 116 out of 120 (96.67%), the percentage of anomalies reported using manual counting fell within the incidence detected using the automatic system. In the remaining comparisons, statistical differences were detected (4 out of 120; 3.33%). Time consumed by the automatic analysis was always higher than the manual one, being influenced by the characteristics of the preparations. CONCLUSION(S): The spot-counting system has potential, but before the service is ready to be offered, we still need to overcome some limitations associated with it.
Authors: Elena García-Mengual; Juan Carlos Triviño; Alba Sáez-Cuevas; Juan Bataller; Miguel Ruíz-Jorro; Xavier Vendrell Journal: J Assist Reprod Genet Date: 2019-01-02 Impact factor: 3.412
Authors: Helen G Tempest; Siu Yan Cheng; David J Gillott; Alan H Handyside; Alan R Thornhill; Darren K Griffin Journal: Asian J Androl Date: 2009-12-28 Impact factor: 3.285
Authors: Mireia Ramos-Muntada; Juan L Trincado; Joan Blanco; Clara Bueno; Virginia C Rodríguez-Cortez; Alex Bataller; Belén López-Millán; Claire Schwab; Margarita Ortega; Pablo Velasco; Maria L Blanco; Josep Nomdedeu; Manuel Ramírez-Orellana; Alfredo Minguela; Jose L Fuster; Esther Cuatrecasas; Mireia Camós; Paola Ballerini; Gabriele Escherich; Judith Boer; Monique DenBoer; Jesús M Hernández-Rivas; Maria J Calasanz; Giovanni Cazzaniga; Christine J Harrison; Pablo Menéndez; Oscar Molina Journal: Mol Oncol Date: 2022-07-19 Impact factor: 7.449