Literature DB >> 1892859

Effect of the antitumour protein alpha-sarcin on the thermotropic behaviour of acid phospholipid vesicles.

M Gasset1, M Oñaderra, A Martínez del Pozo, G P Schiavo, J Laynez, P Usobiaga, J G Gavilanes.   

Abstract

The antitumour protein alpha-sarcin modifies the thermotropic behaviour of phospholipid vesicles. This has been studied by fluorescence depolarization measurements and differential scanning calorimetry. A surface protein-phospholipid interaction is detected by measuring the polarization degree of TMA-DPH-labelled vesicles. At the higher protein/lipid molar ratios studied, the alpha-sarcin-vesicles complexes exhibit different thermotropic behaviour depending on whether they are prepared above or below the Tm of the corresponding phospholipid. Labelling of the protein with photoactive phospholipids has also been considered. alpha-Sarcin penetrates the bilayer deep enough to be labelled with the photoactive group located at the C-12 of the fatty acid acyl chain of phospholipids forming vesicles.

Entities:  

Mesh：

Substances：

Year:  1991        PMID： 1892859     DOI： 10.1016/0005-2736(91)90055-d

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  9 in total

1.  The antifungal protein from Aspergillus giganteus causes membrane permeabilization.

Authors:  T Theis; M Wedde; V Meyer; U Stahl
Journal:  Antimicrob Agents Chemother       Date:  2003-02       Impact factor: 5.191

2.  Translocation of alpha-sarcin across the lipid bilayer of asolectin vesicles.

Authors:  M Oñaderra; J M Mancheño; M Gasset; J Lacadena; G Schiavo; A Martínez del Pozo; J G Gavilanes
Journal:  Biochem J       Date:  1993-10-01       Impact factor: 3.857

3.  Kinetic study of the cytotoxic effect of alpha-sarcin, a ribosome inactivating protein from Aspergillus giganteus, on tumour cell lines: protein biosynthesis inhibition and cell binding.

Authors:  J Turnay; N Olmo; A Jiménez; M A Lizarbe; J G Gavilanes
Journal:  Mol Cell Biochem       Date:  1993-05-12       Impact factor: 3.396

4.  Identification and evaluation of a major cytotoxin of A. fumigatus.

Authors:  T Madan; N Arora; P U Sarma
Journal:  Mol Cell Biochem       Date:  1997-02       Impact factor: 3.396

5.  Membrane interaction of a beta-structure-forming synthetic peptide comprising the 116-139th sequence region of the cytotoxic protein alpha-sarcin.

Authors:  J M Mancheño; M Gasset; J P Albar; J Lacadena; A Martínez del Pozo; M Oñaderra; J G Gavilanes
Journal:  Biophys J       Date:  1995-06       Impact factor: 4.033

6.  Kinetic study of the aggregation and lipid mixing produced by alpha-sarcin on phosphatidylglycerol and phosphatidylserine vesicles: stopped-flow light scattering and fluorescence energy transfer measurements.

Authors:  J M Mancheño; M Gasset; J Lacadena; F Ramón; A Martínez del Pozo; M Oñaderra; J G Gavilanes
Journal:  Biophys J       Date:  1994-09       Impact factor: 4.033

7.  Substitution of histidine-137 by glutamine abolishes the catalytic activity of the ribosome-inactivating protein alpha-sarcin.

Authors:  J Lacadena; J M Mancheño; A Martinez-Ruiz; A Martínez del Pozo; M Gasset; M Oñaderra; J G Gavilanes
Journal:  Biochem J       Date:  1995-07-15       Impact factor: 3.857

Review 8.  Hirsutellin A: A Paradigmatic Example of the Insecticidal Function of Fungal Ribotoxins.

Authors:  Elías Herrero-Galán; Lucía García-Ortega; Miriam Olombrada; Javier Lacadena; Álvaro Martínez Del Pozo; José G Gavilanes; Mercedes Oñaderra
Journal:  Insects       Date:  2013-07-09       Impact factor: 2.769

9.  Interaction of insulin, cholesterol-derivatized mannan, and carboxymethyl chitin with liposomes: A differential scanning calorimetry study.

Authors:  M Tabbakhian; J A Rogers
Journal:  Res Pharm Sci       Date:  2012-01
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.