Literature DB >> 18926890

Enzyme-linked immunosorbent assay for total sennosides using anti-sennside A and anti-sennoside B monoclonal antibodies.

Osamu Morinaga1, Takuhiro Uto, Seiichi Sakamoto, Hiroyuki Tanaka, Yukihiro Shoyama.   

Abstract

Total sennosides concentration is a very important factor when rhubarb and senna will be used as crude drugs. However, one-step analytical technique for total sennosides has not been reported except HPLC. An enzyme-linked immunosorbent assay (ELISA) for total sennosides concentration by using the combination of anti-sennoside A (SA) and anti-sennoside B (SB) monoclonal antibodies (MAbs) in a single assay has been investigated. Total sennosides concentration in rhubarb and senna samples determined by newly developed assay system showed good agreement with those analyzed by ELISA using anti-SA MAb and anti-SB MAb, respectively.

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Year:  2008        PMID： 18926890     DOI： 10.1016/j.fitote.2008.09.004

Source DB:  PubMed          Journal:  Fitoterapia        ISSN： 0367-326X            Impact factor:   2.882


  4 in total

1.  Development of an indirect competitive enzyme-linked immunosorbent assay (icELISA) using highly specific monoclonal antibody against paclitaxel.

Authors:  Zhi Chao; Mingming Tan; Madan Kumar Paudel; Seiichi Sakamoto; Liling Ma; Kaori Sasaki-Tabata; Hiroyuki Tanaka; Yukihiro Shoyama; Lijiang Xuan; Satoshi Morimoto
Journal:  J Nat Med       Date:  2012-09-25       Impact factor: 2.343

2.  Ultrasensitive Time-Resolved Fluoroimmunoassay for Saikosaponin a in Chaihu (Bupleuri Radix).

Authors:  Zhi Chao; Qian Cui; Enwei Tian; Weiping Zeng; Xuan Cai; Xiaolei Li; Hiroyuki Tanaka; Yukihiro Shoyama; Yingsong Wu
Journal:  PLoS One       Date:  2016-03-11       Impact factor: 3.240

Review 3.  Monoclonal Antibodies and Immunoassay for Medical Plant-Derived Natural Products: A Review.

Authors:  Xin Yan; Yan Zhao; Yue Zhang; Huihua Qu
Journal:  Molecules       Date:  2017-02-26       Impact factor: 4.411

Review 4.  Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies.

Authors:  Takuhiro Uto; Tomoe Ohta; Shunsuke Fujii; Yukihiro Shoyama
Journal:  Antibodies (Basel)       Date:  2021-12-06
  4 in total

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