Literature DB >> 1891359

Selection of sequences recognized by a DNA binding protein using a preparative southwestern blot.

A D Keller1, T Maniatis.   

Abstract

We have tested the feasibility of using a preparative scale Southwestern blot to select DNA sequences specifically recognized by a DNA binding protein. We constructed a library of random 15-mer oligonucleotides and screened it using a preparative Southwestern blot with the transcriptional repressor, PRDI-BF1. Several sequences specifically recognized by PRDI-BF1 were isolated. All of these sequences are similar to PRDI, a regulatory element previously demonstrated to bind PRDI-BF1.

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Year:  1991        PMID： 1891359      PMCID： PMC328708          DOI： 10.1093/nar/19.17.4675

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  21 in total

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2.  Differences and similarities in DNA-binding preferences of MyoD and E2A protein complexes revealed by binding site selection.

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3.  A lymphoid-specific protein binding to the octamer motif of immunoglobulin genes.

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Authors:  W K Miskimins; M P Roberts; A McClelland; F H Ruddle
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5.  Whole genome PCR: application to the identification of sequences bound by gene regulatory proteins.

Authors:  K W Kinzler; B Vogelstein
Journal:  Nucleic Acids Res       Date:  1989-05-25       Impact factor: 16.971

6.  Overlapping positive and negative regulatory domains of the human beta-interferon gene.

Authors:  S Goodbourn; T Maniatis
Journal:  Proc Natl Acad Sci U S A       Date:  1988-03       Impact factor: 11.205

7.  Interferon-beta gene regulation: tandemly repeated sequences of a synthetic 6 bp oligomer function as a virus-inducible enhancer.

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8.  Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.

Authors:  F W Studier; B A Moffatt
Journal:  J Mol Biol       Date:  1986-05-05       Impact factor: 5.469

9.  Viral myb oncogene encodes a sequence-specific DNA-binding activity.

Authors:  H Biedenkapp; U Borgmeyer; A E Sippel; K H Klempnauer
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10.  Transformation of bacteria with plasmid DNA by electroporation.

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Journal:  Anal Biochem       Date:  1988-04       Impact factor: 3.365
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  7 in total

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2.  Identification of novel factors that bind to the PRD I region of the human beta-interferon promoter.

Authors:  S T Whiteside; K V Visvanathan; S Goodbourn
Journal:  Nucleic Acids Res       Date:  1992-04-11       Impact factor: 16.971

3.  Isolation of high affinity cellular targets of the embryonal LTR binding protein, an undifferentiated embryonal carcinoma cell-specific repressor of Moloney leukemia virus.

Authors:  T Tsukiyama; O Niwa
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4.  DNA-binding specificity of NGFI-A and related zinc finger transcription factors.

Authors:  A H Swirnoff; J Milbrandt
Journal:  Mol Cell Biol       Date:  1995-04       Impact factor: 4.272

5.  Identification of a nucleic acid-binding region within the largest subunit of Drosophila melanogaster RNA polymerase II.

Authors:  R E Kontermann; M Kobor; E K Bautz
Journal:  Protein Sci       Date:  1993-02       Impact factor: 6.725

6.  Regulation of hepA of Anabaena sp. strain PCC 7120 by elements 5' from the gene and by hepK.

Authors:  J Zhu; R Kong; C P Wolk
Journal:  J Bacteriol       Date:  1998-08       Impact factor: 3.490

7.  An extended set of PRDM1/BLIMP1 target genes links binding motif type to dynamic repression.

Authors:  Gina M Doody; Matthew A Care; Nicholas J Burgoyne; James R Bradford; Maria Bota; Constanze Bonifer; David R Westhead; Reuben M Tooze
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  7 in total

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