Literature DB >> 1891067

Respiratory burst activity in brain macrophages: a flow cytometric study on cultured rat microglia.

R B Banati1, G Rothe, G Valet, G W Kreutzberg.   

Abstract

A new flow cytometric method for the investigation of the respiratory burst of macrophages/microglia isolated from neonatal rat brain has been established. Respiratory burst activity was measured quantitatively in single viable cells by the intracellular oxidation of non-fluorescent dihydrorhodamine 123 (DHR) to fluorescent rhodamine 123. Cultured microglia exhibited high spontaneous respiratory burst activity already before stimulation. After maximal stimulation with phorbol myristate acetate, DHR oxidation rose by 40-95%. The respiratory burst activity in resident or inflammatory, i.e. thioglycolate elicited, peritoneal macrophages was significantly lower than in cultured brain macrophages suggesting a high potential of microglia for oxidative tissue destruction.

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Year:  1991        PMID： 1891067     DOI： 10.1111/j.1365-2990.1991.tb00718.x

Source DB:  PubMed          Journal:  Neuropathol Appl Neurobiol        ISSN： 0305-1846            Impact factor:   8.090


  8 in total

1.  The microglial reaction in the rat hippocampus following global ischemia: immuno-electron microscopy.

Authors:  J Gehrmann; P Bonnekoh; T Miyazawa; U Oschlies; E Dux; K A Hossmann; G W Kreutzberg
Journal:  Acta Neuropathol       Date:  1992       Impact factor: 17.088

2.  A transmission electron microscopic study of microglia/macrophages in the hippocampal cortex and neocortex following chronic exposure to valproate.

Authors:  Maria E Sobaniec-Lotowska
Journal:  Int J Exp Pathol       Date:  2005-04       Impact factor: 1.925

3.  S100A8/A9 Drives Neuroinflammatory Priming and Protects against Anxiety-like Behavior after Sepsis.

Authors:  Scott J Denstaedt; Joanna L Spencer-Segal; Michael W Newstead; Klaudia Laborc; Anne P Zhao; Alexander Hjelmaas; Xianying Zeng; Huda Akil; Theodore J Standiford; Benjamin H Singer
Journal:  J Immunol       Date:  2018-03-21       Impact factor: 5.422

4.  Differential response of neural cells to trauma-induced free radical production in vitro.

Authors:  K S Panickar; A R Jayakumar; M D Norenberg
Journal:  Neurochem Res       Date:  2002-02       Impact factor: 3.996

5.  Inflammatory mediators and activity of human peritoneal macrophages.

Authors:  W M Pruimboom; A P van Dijk; C J Tak; F J Zijlstra; I L Bonta; J H Wilson
Journal:  Agents Actions       Date:  1993

6.  DNA damage induced by tumour necrosis factor-alpha in L929 cells is mediated by mitochondrial oxygen radical formation.

Authors:  Y Shoji; Y Uedono; H Ishikura; N Takeyama; T Tanaka
Journal:  Immunology       Date:  1995-04       Impact factor: 7.397

7.  Peritoneal macrophages from patients with cirrhotic ascites show impaired phagocytosis and vigorous respiratory burst.

Authors:  Abdel Motaal M Ahmed; Adrian Bomford; Kayhan T Nouri-Aria; Ted Davies; Roger Smith; Roger Williams
Journal:  Results Immunol       Date:  2011-09-01

Review 8.  The Translocator Protein (TSPO) in Mitochondrial Bioenergetics and Immune Processes.

Authors:  Calina Betlazar; Ryan J Middleton; Richard Banati; Guo-Jun Liu
Journal:  Cells       Date:  2020-02-24       Impact factor: 6.600
  8 in total

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