Literature DB >> 1890179

Blocking enzyme-linked immunosorbent assay for detection of antibodies to Actinobacillus pleuropneumoniae serotype 2.

R Nielsen1, T Plambeck, N T Foged.   

Abstract

A blocking enzyme-linked immunosorbent assay (ELISA), based upon a polyclonal rabbit antiserum specific to Actinobacillus pleuropneumoniae serotype 2, was developed for the detection of antibodies to A. pleuropneumoniae serotype 2 in pigs. By testing sera from pigs experimentally infected with the 11 recognized serotypes of A. pleuropneumoniae, the assay was proven to be specific for A. pleuropneumoniae serotype 2. With field sera from herds infected with A. pleuropneumoniae serotype 2, the assay was found to be more sensitive than the complement fixation test. Positive results were not observed with field sera from herds known to be free from Actinobacillus infection or with sera from two herds infected with either A. pleuropneumoniae serotype 6 or 8. The high diagnostic sensitivity and specificity of the blocking ELISA will make it useful in field diagnostic work.

Entities:  

Mesh：

Substances：

Year:  1991        PMID： 1890179      PMCID： PMC269872          DOI： 10.1128/jcm.29.4.794-797.1991

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Seroepidemiology of Actinobacillus pleuropneumoniae.

Authors:  R Nielsen
Journal:  Can Vet J       Date:  1988-07       Impact factor: 1.008

2.  The Use of an Enzyme-linked Immunosorbent Assay for Diagnosis of Actinobacillus pleuropneumoniae Infection in Pigs.

Authors:  P J Willson; C Schipper; E D Morgan
Journal:  Can Vet J       Date:  1988-07       Impact factor: 1.008

3.  Bacteriological, serological, and pathological examinations of Haemophilus pleuropneumoniae infection in 200 slaughtered pigs.

Authors:  K Kume; I Nagano; T Nakai
Journal:  Nihon Juigaku Zasshi       Date:  1986-10

4.  [Haemophilus infection in pigs. 3. Serological studies on Haemophilus parahaemolyticus].

Authors:  J Nicolet
Journal:  Zentralbl Bakteriol Orig       Date:  1971

Review 5.  Haemophilus pleuropneumoniae infection in swine: a review.

Authors:  T N Sebunya; J R Saunders
Journal:  J Am Vet Med Assoc       Date:  1983-06-15       Impact factor: 1.936

6.  Serological characterization of 8 Haemophilus pleuropneumoniae strains and proposal of a new serotype: serotype 8.

Authors:  R Nielsen; P J O'Connor
Journal:  Acta Vet Scand       Date:  1984       Impact factor: 1.695

7.  An enzyme-linked immunosorbent assay, using an EDTA-extracted antigen for the serology of Haemophilus pleuropneumoniae.

Authors:  J Nicolet; P Paroz; M Krawinkler; A Baumgartner
Journal:  Am J Vet Res       Date:  1981-12       Impact factor: 1.156

8.  Immune response to an Actinobacillus pleuropneumoniae vaccine in swine.

Authors:  P Rousseau; R Assaf; G Boulay; M Désy
Journal:  Can Vet J       Date:  1988-12       Impact factor: 1.008
  8 in total
  11 in total

1.  Antibody response in sows and piglets following vaccination against Mycoplasma hyopneumoniae, toxigenic Pasteurella multocida, and Actinobacillus pleuropneumoniae.

Authors:  Charlotte S Kristensen; Margit Andreasen; Annette K Ersbøll; Jens P Nielsen
Journal:  Can J Vet Res       Date:  2004-01       Impact factor: 1.310

2.  Serological characterization of Actinobacillus pleuropneumoniae biotype 1 strains antigenically related to both serotypes 2 and 7.

Authors:  R Nielsen; L O Andresen; T Plambeck
Journal:  Acta Vet Scand       Date:  1996       Impact factor: 1.695

3.  A novel enzyme-linked immunosorbent assay using the recombinant Actinobacillus pleuropneumoniae ApxII antigen for diagnosis of pleuropneumonia in pig herds.

Authors:  G Leiner; B Franz; K Strutzberg; G F Gerlach
Journal:  Clin Diagn Lab Immunol       Date:  1999-07

4.  Determination of the optimal cutoff value for a serological assay: an example using the Johne's Absorbed EIA.

Authors:  S E Ridge; A L Vizard
Journal:  J Clin Microbiol       Date:  1993-05       Impact factor: 5.948

5.  Clonal analysis of the Actinobacillus pleuropneumoniae population in a geographically restricted area by multilocus enzyme electrophoresis.

Authors:  K Møller; R Nielsen; L V Andersen; M Kilian
Journal:  J Clin Microbiol       Date:  1992-03       Impact factor: 5.948

6.  Evaluation of 5' nuclease assay for detection of Actinobacillus pleuropneumoniae.

Authors:  O Angen; J Jensen; D T Lavritsen
Journal:  J Clin Microbiol       Date:  2001-01       Impact factor: 5.948

7.  Serodiagnosis of swine pleuropneumonia due to Actinobacillus pleuropneumoniae serotypes 7 and 4 using long-chain lipopolysaccharides.

Authors:  M Gottschalk; E Altman; S Lacouture; F De Lasalle; J D Dubreuil
Journal:  Can J Vet Res       Date:  1997-01       Impact factor: 1.310

8.  Detection of antibodies against Actinobacillus pleuropneumoniae serotype 5 using an inhibition enzyme immunoassay.

Authors:  E I Stenbaek; F De LaSalle; M Gottschalk
Journal:  Can J Vet Res       Date:  1997-01       Impact factor: 1.310

9.  Identification and characterization of serovar-independent immunogens in Actinobacillus pleuropneumoniae.

Authors:  Fabio Antenucci; Cyrielle Fougeroux; Janine T Bossé; Zofia Magnowska; Camille Roesch; Paul Langford; Peter Johannes Holst; Anders Miki Bojesen
Journal:  Vet Res       Date:  2017-11-09       Impact factor: 3.683

10.  Polymorphism analysis of the apxIA gene of Actinobacillus pleuropneumoniae serovar 5 isolated in swine herds from Brazil.

Authors:  Lucas Fernando Dos Santos; Richard Costa Polveiro; Thalita Scatamburlo Moreira; Pedro Marcus Pereira Vidigal; Yung-Fu Chang; Maria Aparecida Scatamburlo Moreira
Journal:  PLoS One       Date:  2018-12-18       Impact factor: 3.240
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.