Literature DB >> 1888038

Quantitative study of protein association at picomolar concentrations: the lambda phage cl repressor.

D Beckett1, K S Koblan, G K Ackers.   

Abstract

A method has been developed for radiolabeling the lambda cl repressor to a specific activity sufficiently high to permit accurate quantitation of the protein in the picomolar range of concentration. Procedures are described whereby the labeled protein can be used for accurate quantitative study of the energetics of repressor assembly by large zone analytical gel chromatography. This methodology is applicable to other systems in which the stoichiometry and energetics of tightly associating DNA binding proteins are currently difficult to measure.

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Year:  1991        PMID： 1888038     DOI： 10.1016/0003-2697(91)90118-d

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  11 in total

1.  DNA looping provides stability and robustness to the bacteriophage lambda switch.

Authors:  Marco J Morelli; Pieter Rein Ten Wolde; Rosalind J Allen
Journal:  Proc Natl Acad Sci U S A       Date:  2009-05-01       Impact factor: 11.205

2.  The lysis-lysogeny decision of bacteriophage 933W: a 933W repressor-mediated long-distance loop has no role in regulating 933W P(RM) activity.

Authors:  Tammy J Bullwinkle; Gerald B Koudelka
Journal:  J Bacteriol       Date:  2011-05-06       Impact factor: 3.490

3.  Changing the mechanism of transcriptional activation by phage lambda repressor.

Authors:  M Li; W R McClure; M M Susskind
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-15       Impact factor: 11.205

Review 4.  Protein-protein interactions: methods for detection and analysis.

Authors:  E M Phizicky; S Fields
Journal:  Microbiol Rev       Date:  1995-03

5.  Electrostatic forces contribute to interactions between trp repressor dimers.

Authors:  K S Martin; C A Royer; K P Howard; J Carey; Y C Liu; K Matthews; E Heyduk; J C Lee
Journal:  Biophys J       Date:  1994-04       Impact factor: 4.033

6.  Transcription of the gene mediating methicillin resistance in Staphylococcus aureus (mecA) is corepressed but not coinduced by cognate mecA and beta-lactamase regulators.

Authors:  T K McKinney; V K Sharma; W A Craig; G L Archer
Journal:  J Bacteriol       Date:  2001-12       Impact factor: 3.490

7.  Evidence for involvement of the C-terminal domain in the dimerization of the CopY repressor protein from Enterococcus hirae.

Authors:  Kristina O Pazehoski; Paul A Cobine; Donald J Winzor; Charles T Dameron
Journal:  Biochem Biophys Res Commun       Date:  2011-02-03       Impact factor: 3.575

8.  Spectral enhancement of proteins: biological incorporation and fluorescence characterization of 5-hydroxytryptophan in bacteriophage lambda cI repressor.

Authors:  J B Ross; D F Senear; E Waxman; B B Kombo; E Rusinova; Y T Huang; W R Laws; C A Hasselbacher
Journal:  Proc Natl Acad Sci U S A       Date:  1992-12-15       Impact factor: 11.205

9.  Determinants of bacteriophage 933W repressor DNA binding specificity.

Authors:  Tammy J Bullwinkle; Daniel Samorodnitsky; Rayna C Rosati; Gerald B Koudelka
Journal:  PLoS One       Date:  2012-04-03       Impact factor: 3.240

10.  Mechanisms that Determine the Differential Stability of Stx⁺ and Stx(-) Lysogens.

Authors:  Michael P Colon; Dolonchapa Chakraborty; Yonatan Pevzner; Gerald B Koudelka
Journal:  Toxins (Basel)       Date:  2016-03-31       Impact factor: 4.546
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