Literature DB >> 1888021

Miniaturization of three carbohydrate analyses using a microsample plate reader.

J D Fox1, J F Robyt.   

Abstract

Three carbohydrate analyses (reducing value by copper-bicinchoninate, total carbohydrate by phenol-sulfuric acid, and D-glucose by glucose oxidase) have been miniaturized using a microsample plate reader. The use of the reducing-value procedure to measure the hydrolysis of starch by alpha-amylase and the use of the glucose oxidase method to measure the hydrolysis of lactose by lactase are illustrated.

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Year:  1991        PMID: 1888021     DOI: 10.1016/0003-2697(91)90300-i

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  48 in total

1.  pH-dependence of the fast step of maltose hydrolysis catalysed by glucoamylase G1 from Aspergillus niger.

Authors:  U Christensen
Journal:  Biochem J       Date:  2000-07-15       Impact factor: 3.857

2.  Enzymatic analysis of an amylolytic enzyme from the hyperthermophilic archaeon Pyrococcus furiosus reveals its novel catalytic properties as both an alpha-amylase and a cyclodextrin-hydrolyzing enzyme.

Authors:  Sung-Jae Yang; Hee-Seob Lee; Cheon-Seok Park; Yong-Ro Kim; Tae-Wha Moon; Kwan-Hwa Park
Journal:  Appl Environ Microbiol       Date:  2004-10       Impact factor: 4.792

3.  Structural insight into the bifunctional mechanism of the glycogen-debranching enzyme TreX from the archaeon Sulfolobus solfataricus.

Authors:  Eui-Jeon Woo; Seungjae Lee; Hyunju Cha; Jong-Tae Park; Sei-Mee Yoon; Hyung-Nam Song; Kwan-Hwa Park
Journal:  J Biol Chem       Date:  2008-08-14       Impact factor: 5.157

4.  Direct Loading of the purified endogenous inhibitor into the cytoplasm of patched cardiomyocytes blocks the ion currents and calcium transport through the NCX1 protein.

Authors:  Liron Boyman; Reuben Hiller; W Jonathan Lederer; Daniel Khananshvili
Journal:  Biochemistry       Date:  2008-06-24       Impact factor: 3.162

5.  Echinacea purpurea (L.) Moench modulates human T-cell cytokine response.

Authors:  Fabiana N Fonseca; Genovefa Papanicolaou; Hong Lin; Clara B S Lau; Edward J Kennelly; Barrie R Cassileth; Susanna Cunningham-Rundles
Journal:  Int Immunopharmacol       Date:  2014-01-13       Impact factor: 4.932

6.  Characterization of SU1 isoamylase, a determinant of storage starch structure in maize.

Authors:  A Rahman; K s Wong; J l Jane; A M Myers; M G James
Journal:  Plant Physiol       Date:  1998-06       Impact factor: 8.340

7.  A comparison of sugar indicators enables a universal high-throughput sugar-1-phosphate nucleotidyltransferase assay.

Authors:  Rocco Moretti; Jon S Thorson
Journal:  Anal Biochem       Date:  2008-03-15       Impact factor: 3.365

8.  Properties of a novel thermostable glucoamylase from the hyperthermophilic archaeon Sulfolobus solfataricus in relation to starch processing.

Authors:  Mi-Sun Kim; Jong-Tae Park; Young-Wan Kim; Hee-Seob Lee; Rose Nyawira; Hyoun-Seung Shin; Cheon-Seok Park; Sang-Ho Yoo; Yong-Ro Kim; Tae-Wha Moon; Kwan-Hwa Park
Journal:  Appl Environ Microbiol       Date:  2004-07       Impact factor: 4.792

9.  Characterization of pullulanase (PUL)-deficient mutants of rice (Oryza sativa L.) and the function of PUL on starch biosynthesis in the developing rice endosperm.

Authors:  Naoko Fujita; Yoshiko Toyosawa; Yoshinori Utsumi; Toshiyuki Higuchi; Isao Hanashiro; Akira Ikegami; Sayuri Akuzawa; Mayumi Yoshida; Akiko Mori; Kotaro Inomata; Rumiko Itoh; Akio Miyao; Hirohiko Hirochika; Hikaru Satoh; Yasunori Nakamura
Journal:  J Exp Bot       Date:  2009-02-03       Impact factor: 6.992

10.  Development of a fluorescence-based method for monitoring glucose catabolism and its potential use in a biomass hydrolysis assay.

Authors:  Lisa J Haney; James G Coors; Aaron J Lorenz; D Raj Raman; Robert P Anex; M Paul Scott
Journal:  Biotechnol Biofuels       Date:  2008-11-19       Impact factor: 6.040

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