A reliable technique for chronic carotid arterial catheterization in the rat.

A reliable and simple method for cannulating the carotid artery of rats is described. The rat was anesthetized with halothane. The right carotid artery located between the omohyoideus and sternohyoideus muscles was exposed through a 2-cm ventral neck incision. The catheter was made of Silastic tubing and a monofilament line, which served as an obturator and internal support. The line was then filled with a viscous mixture of heparin, polyvinylpyrrolidinone and normal saline to prevent the formation of blood clots. The catheter was advanced through the carotid artery towards the heart by a predetermined distance (15-20 mm) depending on the size of the rat. The catheter was well tolerated by the rats and the success rate was 95%. Its patency lasted for at least 7 days postsurgery without any special maintenance care. With the described method one would be able to perform repetitive blood sampling and arterial blood pressure measurements in unanesthetized and unrestrained rats for prolonged period after catheterization.