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Literature DB >> 18852851

Evaluating assay precision.

Abstract

* When evaluating the precision of a method it is necessary to assess the repeatability (within-run) and the total or within-laboratory precision. * It is insufficient to assess repeatability in a single run. * Clinical and Laboratory Standards Institute (CLSI) document EP05-A2 describes the protocols for determining the precision of a method. The precision of a method should be tested at at-least two levels; each run in duplicate, with two runs per day over 20 days. CLSI document EP15-A2 describes the protocols that should be undertaken by the user to verify precision claims by a manufacturer. Precision claims by a manufacturer should be tested at at-least two levels, by running three replicates over five days. * A spreadsheet for assisting with the calculations described in this article is available from the AACB web-site.

Year: 2008 PMID： 18852851 PMCID： PMC2556577

Source DB: PubMed Journal: Clin Biochem Rev ISSN： 0159-8090

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Cited

39 in total

1. Real-time quantitative reverse transcription PCR for monitoring of blood-stage Plasmodium falciparum infections in malaria human challenge trials.

Authors: Sean C Murphy; Jennifer L Prentice; Kathryn Williamson; Carolyn K Wallis; Ferric C Fang; Michal Fried; Cris Pinzon; Ruobing Wang; Angela K Talley; Stefan H I Kappe; Patrick E Duffy; Brad T Cookson
Journal: Am J Trop Med Hyg Date: 2012-03 Impact factor: 2.345

2. Rapid quantitative chiral amphetamines liquid chromatography-tandem mass spectrometry: method in plasma and oral fluid with a cost-effective chiral derivatizing reagent.

Authors: Matthew N Newmeyer; Marta Concheiro; Marilyn A Huestis
Journal: J Chromatogr A Date: 2014-07-05 Impact factor: 4.759

Evaluating assay precision.

1. Real-time quantitative reverse transcription PCR for monitoring of blood-stage Plasmodium falciparum infections in malaria human challenge trials.

2. Rapid quantitative chiral amphetamines liquid chromatography-tandem mass spectrometry: method in plasma and oral fluid with a cost-effective chiral derivatizing reagent.

3. Evaluation of imprecision, bias and total error of clinical chemistry analysers.

4. EP15A3 Based Precision and Trueness Verification of VITROS HbA1C Immunoassay.

5. Evaluation of the Aptima HCV Quant Dx Assay Using Serum and Dried Blood Spots.

6. Morphine and codeine in oral fluid after controlled poppy seed administration.

7. Urinary levels of the acrolein conjugates of carnosine are associated with inhaled toxicants.

8. Coating and Pharmacokinetic Evaluation of Air Spray Coated Drug Coated Balloons.

9. A high-throughput screening assay of ascorbate in brain samples.

10. Clinical evaluation of a single-reaction real-time RT-PCR for pan-dengue and chikungunya virus detection.